Alizarin red S–zinc(II) fluorescent ensemble for selective detection of hydrogen sulphide and assay with an H2S donor

Rahul Kaushik; Pawan Kumar; Amrita Ghosh; Neha Gupta; Davinder Kaur; Saroj Arora; D. Amilan Jose

doi:10.1039/C5RA11901D

Alizarin red S–zinc(ii) fluorescent ensemble for selective detection of hydrogen sulphide and assay with an H₂S donor†

Rahul Kaushik,^a Pawan Kumar,^a Amrita Ghosh,^a Neha Gupta,^b Davinder Kaur,^c Saroj Arora^c and D. Amilan Jose*^a

* Corresponding authors

^a Department of Chemistry, NIT-Kurukshetra, Haryana-136119, India
E-mail: amilanjosenit@nitkkr.ac.in
Tel: +91-1744233559

^b Department of Chemistry, Guru Nanak Dev University, Amritsar, India

^c Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India

Abstract

A new Alizarin Red S based fluorescent probe ARS–Zn(II) has been reported for the detection of H₂S in aqueous buffer solution. In the presence of zinc ions, ARS displayed an increase in fluorescence intensity through the formation of an ARS–Zn(II) ensemble. The addition of H₂S to the ensemble caused the disassembly of the metal and ARS, which restored the fluorescence intensity of ARS. This sensing ensemble exhibited H₂S-selectivity over other biothiols and biologically relevant analytes. The calculated detection limit of ARS–Zn(II) with H₂S was 92 nM. ARS–Zn(II) also detected H₂S in serum under physiological conditions. Moreover, as a practical application, it could be used for the real time monitoring of H₂S released from the H₂S-donor molecule benzoic (methyl carbonic) dithioperoxyanhydride and also applied to live cell imaging. The reported ensemble ARS–Zn(II) has advantages like ready availability, high selectivity with good sensitivity, fast response time, higher excitation (520 nm) and emission (625 nm) wavelengths and real time fluorescence assay with H₂S donors.

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Article information

DOI: https://doi.org/10.1039/C5RA11901D
Article type: Paper
Submitted: 20 Jun 2015
Accepted: 07 Sep 2015
First published: 08 Sep 2015

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RSC Adv., 2015,5, 79309-79316

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Alizarin red S–zinc(II) fluorescent ensemble for selective detection of hydrogen sulphide and assay with an H₂S donor

R. Kaushik, P. Kumar, A. Ghosh, N. Gupta, D. Kaur, S. Arora and D. A. Jose, RSC Adv., 2015, 5, 79309 DOI: 10.1039/C5RA11901D

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