Issue 52, 2018

Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics

Abstract

Here, we developed two pairs of high-contrast chemical probes and their RNA aptamers with distinct readout channels that permitted simultaneous live-cell imaging of endogenous β-actin and cortactin mRNAs. Application of this technology allowed the direct observation of the formation process of stress granules, protein–RNA assemblies essential for cellular response to the environment.

Graphical abstract: Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics

Supplementary files

Article information

Article type
Communication
Submitted
11 May 2018
Accepted
01 Jun 2018
First published
02 Jun 2018

Chem. Commun., 2018,54, 7151-7154

Author version available

Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics

K. Yatsuzuka, S. Sato, K. B. Pe, Y. Katsuda, I. Takashima, M. Watanabe and M. Uesugi, Chem. Commun., 2018, 54, 7151 DOI: 10.1039/C8CC03805H

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