Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

Fei Deng; Yi Li; Md Jakir Hossain; Michael D. Kendig; Ria Arnold; Ewa M. Goldys; Margaret J. Morris; Guozhen Liu

doi:10.1039/C9AN01300H

Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood†

Fei Deng,^ab Yi Li,^ab Md Jakir Hossain,^c Michael D. Kendig,^c Ria Arnold,^c Ewa M. Goldys,

^ab Margaret J. Morris^c and Guozhen Liu

*^ab

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* Corresponding authors

^a Graduate School of Biomedical Engineering, ARC Centre of Excellence in Nanoscale Biophotonics, Faculty of Engineering, UNSW Sydney, NSW 2052, Australia
E-mail: guozhen.liu@unsw.edu.au
Tel: +61-2-9385 0714

^b Australian Centre for NanoMedicine, UNSW Sydney, NSW 2052, Australia

^c School of Medical Sciences, UNSW Sydney, NSW 2052, Australia

Abstract

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL⁻¹, and a linear detection range of 12.5–200 pg mL⁻¹. Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.

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DOI: https://doi.org/10.1039/C9AN01300H
Article type: Paper
Submitted: 11 Jul 2019
Accepted: 08 Aug 2019
First published: 13 Aug 2019

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Analyst, 2019,144, 5682-5690

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Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

F. Deng, Y. Li, M. J. Hossain, M. D. Kendig, R. Arnold, E. M. Goldys, M. J. Morris and G. Liu, Analyst, 2019, 144, 5682 DOI: 10.1039/C9AN01300H

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Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood†

Abstract

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Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

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