Issue 65, 2020
From the journal:

Chemical Communications

The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases†

Yujing Hu,ab   Weihua Xu,a   Chenggong Hui,c   Jian Xu,a   Meilan Huang, ORCID logo *c   Xianfu Lina  and  Qi Wu ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Zhejiang University, Hangzhou 310027, China
E-mail: llc123@zju.edu.cn

b College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, P. R. China

c School of Chemistry and Chemical Engineering, Queen's University, Belfast, UK
E-mail: m.huang@qub.ac.uk

Abstract

The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).

Graphical abstract: The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

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Article information

DOI
https://doi.org/10.1039/D0CC03721D
Article type
Communication
Submitted
26 May 2020
Accepted
08 Jul 2020
First published
08 Jul 2020

Chem. Commun., 2020,56, 9356-9359

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The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

Y. Hu, W. Xu, C. Hui, J. Xu, M. Huang, X. Lin and Q. Wu, Chem. Commun., 2020, 56, 9356 DOI: 10.1039/D0CC03721D

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