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Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

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Abstract

Three porphyrin systems have been characterised for use in two-photon fluorescence imaging of biological samples. We have determined the two-photon absorption cross sections (σ2) of the di-cation, free-base and metallated forms of hematoporphyrin derivative (HpD), hematoporphyrin IX (Hp9) and a boronated protoporphyrin (BOPP) using the open-aperture Z-scan and the two-photon induced fluorescence (TPIF) techniques at an excitation wavelength of 800 nm. The insertion of either protons or a metal ion into the macrocycle is shown not to significantly influence the σ2 of the porphyrins. Two-photon time-resolved fluorescence images of C6 glioma cells transfected with a free-base form of the BOPP have been obtained as a function of the porphyrin concentration. These studies reveal a maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 µg mL−1.

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Authors and Affiliations

  1. School of Chemistry, The University of Melbourne, Victoria, 3010, Australia

    Sean Mathai, Damian K. Bird, Trevor A. Smith & Kenneth P. Ghiggino

  2. Department of Surgery, Royal Melbourne Hospital, Parkville, 3052, Australia

    Stan S. Stylli

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  1. Sean Mathai
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  2. Damian K. Bird
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  3. Stan S. Stylli
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  4. Trevor A. Smith
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  5. Kenneth P. Ghiggino
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Correspondence to Trevor A. Smith.

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This paper was published as part of the special issue in honour of David Phillips.

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Mathai, S., Bird, D.K., Stylli, S.S. et al. Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers. Photochem Photobiol Sci 6, 1019–1026 (2007). https://doi.org/10.1039/b705101h

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