Issue 6, 2010
From the journal:

Analyst

Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa†

Konrad Meister,a   Diedrich A. Schmidt,a   Erik Bründermann*a  and  Martina Havenitha  

* Corresponding authors

a Ruhr-Universität Bochum, Physical Chemistry II, Bldg. NC 7/72, Universitätsstr. 150, Bochum, Germany
E-mail: erik.bruendermann@rub.de
Fax: +49 2343214183
Tel: +49 2343224239

Abstract

Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.

Graphical abstract: Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B927012D
Article type
Paper
Submitted
22 Dec 2009
Accepted
24 Mar 2010
First published
13 Apr 2010

Analyst, 2010,135, 1370-1374

Permissions

Request permissions

Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

K. Meister, D. A. Schmidt, E. Bründermann and M. Havenith, Analyst, 2010, 135, 1370 DOI: 10.1039/B927012D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements