Issue 1, 2012
From the journal:

Journal of Analytical Atomic Spectrometry

Improving acquisition times of elemental bio-imaging for quadrupole-based LA-ICP-MS†

Jessica Lear,a   Dominc Hare,a   Paul Adlard,bc   David Finkelstein‡bc  and  Philip Doble‡*a  

* Corresponding authors

a Elemental Bio-Imaging Facility, University of Technology Sydney, Broadway, Sydney, NSW, Australia
E-mail: Philip.Doble@uts.edu.au
Fax: +61 2 9514 1460
Tel: +61 2 9514 1792

b The Mental Health Research Institute, Parkville, Australia

c Centre for Neuroscience, University of Melbourne, Parkville, Australia

Abstract

Elemental bio-imaging experiments by quadrupole-based LA-ICP-MS normally employ scan speeds where the distance traversed in one second is equal to or less than the diameter of the laser beam. Consequently, data for a higher-resolution (pixel size = 15 μm2) image of a 5 mm2 tissue section can take upwards of 30 h to acquire. Appropriate laser scan speeds may be calculated by consideration of the relationship between laser scan speed, laser spot diameter and the total scan cycle of the quadrupole mass analyser. This paper presents a simple method to calculate the laser scan speeds capable of reducing the acquisition time by up to a factor of 5 whilst maintaining dimensional integrity of the image.

Graphical abstract: Improving acquisition times of elemental bio-imaging for quadrupole-based LA-ICP-MS

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Article information

DOI
https://doi.org/10.1039/C1JA10301F
Article type
Technical Note
Submitted
07 Oct 2011
Accepted
10 Oct 2011
First published
28 Oct 2011

J. Anal. At. Spectrom., 2012,27, 159-164

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Improving acquisition times of elemental bio-imaging for quadrupole-based LA-ICP-MS

J. Lear, D. Hare, P. Adlard, D. Finkelstein and P. Doble, J. Anal. At. Spectrom., 2012, 27, 159 DOI: 10.1039/C1JA10301F

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