Issue 4, 2012
From the journal:

Journal of Analytical Atomic Spectrometry

CellSpace: A module for creating spatially registered laser ablation images within the Iolite freeware environment

Bence Paul,*ab   Chad Paton,c   Ashley Norris,d   Jon Woodhead,a   John Hellstrom,a   Janet Hergta  and  Alan Greiga  

* Corresponding authors

a School of Earth Sciences, The University of Melbourne, Parkville, Vic 3010, Australia
E-mail: bpaul@unimelb.edu.au

b CODES ARC Centre of Excellence in Ore Deposits and School of Earth Sciences, University of Tasmania, Tasmania, Australia

c Centre for Star and Planet Formation, Geological Museum, University of Copenhagen, Øster Voldgade 5-7, DK-1350 Copenhagen, Denmark

d Resonetics LLC, Nashua, New Hampshire, United States of America

Abstract

We present a novel approach to creating compositional images using a module created for use with the freely distributed software package Iolite. The module creates images by synchronising the state of the laser (e.g., whether the laser is firing or not) and the position on the sample, which are recorded in laser log files, with concurrently collected mass spectrometer data. When these two data sources are synchronised, mass spectrometer data which are recorded temporally can then be displayed versus ablation position (i.e., spatially). Each mass spectrometer reading is then plotted as a circular spot representing the size of the area ablated. This approach has many advantages. CellSpace takes advantage of Iolite's ability to manipulate data from various mass spectrometers and to reduce data of different types. Laser ablation data can be plotted over other images, such as those produced by scanning electron microscopes, where the image has been transformed into cell coordinates using third party software. This allows the analyst to visualise laser ablation data in context and to correlate sample data from multiple sources and/or techniques. The code also has the advantage of averaging data spatially, rather than just temporally, and faithfully presents the data as a corresponding laser spot, rather than a simple rectangular pixel. Here we provide an example of a fish otolith, where trace element concentrations and Sr-isotopic compositions are overlain on microscope images, providing information on migration patterns that are applicable to population studies and fisheries conservation.

Graphical abstract: CellSpace: A module for creating spatially registered laser ablation images within the Iolite freeware environment

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Article information

DOI
https://doi.org/10.1039/C2JA10383D
Article type
Technical Note
Submitted
22 Dec 2011
Accepted
17 Feb 2012
First published
20 Feb 2012

J. Anal. At. Spectrom., 2012,27, 700-706

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CellSpace: A module for creating spatially registered laser ablation images within the Iolite freeware environment

B. Paul, C. Paton, A. Norris, J. Woodhead, J. Hellstrom, J. Hergt and A. Greig, J. Anal. At. Spectrom., 2012, 27, 700 DOI: 10.1039/C2JA10383D

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