Issue 87, 2013
From the journal:

Chemical Communications

Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry†

Fazel Shabanpoora  and  Michael J. Gait*a  

* Corresponding authors

a MRC Laboratory of Molecular Biology, Cambridge, UK
E-mail: mgait@mrc-lmb.cam.ac.uk

Abstract

We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay.

Graphical abstract: Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

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Article information

DOI
https://doi.org/10.1039/C3CC46067C
Article type
Communication
Submitted
08 Aug 2013
Accepted
12 Sep 2013
First published
13 Sep 2013
This article is Open Access
Creative Commons BY license

Chem. Commun., 2013,49, 10260-10262

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Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

F. Shabanpoor and M. J. Gait, Chem. Commun., 2013, 49, 10260 DOI: 10.1039/C3CC46067C

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