Issue 14, 2014
From the journal:

Analyst

Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction†

Dmitriy A. Khodakov,*a   Anastasia S. Khodakova,b   Adrian Linacreb  and  Amanda V. Ellis*a  

* Corresponding authors

a Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, SA, 5001 Australia
E-mail: dmitriy.khodakov@flinders.edu.au, amanda.ellis@fliders.edu.au

b School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001 Australia

Abstract

This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

Graphical abstract: Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

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Article information

DOI
https://doi.org/10.1039/C4AN00694A
Article type
Communication
Submitted
17 Apr 2014
Accepted
15 May 2014
First published
15 May 2014

Analyst, 2014,139, 3548-3551

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Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

D. A. Khodakov, A. S. Khodakova, A. Linacre and A. V. Ellis, Analyst, 2014, 139, 3548 DOI: 10.1039/C4AN00694A

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