Issue 7, 2016
From the journal:

Organic & Biomolecular Chemistry

Blue ‘turn-on’ fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosa biofilms†

Mina Barzegar Amiri Olia,ab   Athanasios Zavras, ORCID logo ab   Carl H. Schiesser*ab  and  Stefanie-Ann Alexanderab  

* Corresponding authors

a ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Australia

b School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, Australia
E-mail: carlhs@unimelb.edu.au
Fax: +61 3 9347 8189
Tel: +61 3 8344 2432

Abstract

Two novel cell-permeable and bacteria-compatible ‘turn-on’ fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440–490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize and quantify free radical and redox processes, and the Cu(II)-complexed coumarin-based probe (16a) was developed for NO detection. Confocal laser-scanning microscopy and subsequent digital analysis of Pseudomonas aeruginosa biofilms stained with 11 or 16a determined that free radical and redox processes and NO generation occur predominantly in live cells during normal biofilm growth.

Graphical abstract: Blue ‘turn-on’ fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosa biofilms

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Article information

DOI
https://doi.org/10.1039/C5OB02441B
Article type
Paper
Submitted
29 Nov 2015
Accepted
14 Jan 2016
First published
15 Jan 2016

Org. Biomol. Chem., 2016,14, 2272-2281

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Blue ‘turn-on’ fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosa biofilms

M. Barzegar Amiri Olia, A. Zavras, C. H. Schiesser and S. Alexander, Org. Biomol. Chem., 2016, 14, 2272 DOI: 10.1039/C5OB02441B

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