Exploration of a library of triazolothiadiazole and triazolothiadiazine compounds as a highly potent and selective family of cholinesterase and monoamine oxidase inhibitors: design, synthesis, X-ray diffraction analysis and molecular docking studies

Imtiaz Khan; Syeda Mahwish Bakht; Aliya Ibrar; Saba Abbas; Shahid Hameed; Jonathan M. White; Usman Ali Rana; Sumera Zaib; Mohammad Shahid; Jamshed Iqbal

doi:10.1039/C5RA00906E

Exploration of a library of triazolothiadiazole and triazolothiadiazine compounds as a highly potent and selective family of cholinesterase and monoamine oxidase inhibitors: design, synthesis, X-ray diffraction analysis and molecular docking studies†

Imtiaz Khan,‡^a Syeda Mahwish Bakht,^b Aliya Ibrar,^a Saba Abbas,^b Shahid Hameed,*^a Jonathan M. White,^c Usman Ali Rana,^d Sumera Zaib,^b Mohammad Shahid^e and Jamshed Iqbal*^b

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* Corresponding authors

^a Department of Chemistry, Quaid-i-Azam University, Islamabad-45320, Pakistan
E-mail: shameed@qau.edu.pk
Fax: +92-51-90642241
Tel: +92-51-90642133

^b Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan
E-mail: drjamshed@ciit.net.pk
Fax: +92-992-383441
Tel: +92-992-383591

^c School of Chemistry and Bio-21 Institute, University of Melbourne, Parkville-3052, Australia

^d Sustainable Energy Technologies (SET) Center, College of Engineering, King Saud University, PO-Box 800, Riyadh 11421, Saudi Arabia

^e German Center for Neurodegenerative Diseases (DZNE), Germany

Abstract

There is a high demand for the collection of small organic molecules (especially N-heterocycles) with diversity and complexity in the process of drug discovery. This need for privileged scaffolds in medicinal research gives an impetus for the development of nitrogen-containing compounds which are widely encountered in natural products, drugs and pharmaceutically active compounds. In this context, a diverse library of new triazolothiadiazole (4a–l) and triazolothiadiazine (5a–p) compounds was designed, synthesized and evaluated as potent and selective inhibitors of electric eel acetylcholinesterase (EeAChE) and horse serum butyrylcholinesterase (hBChE) by Ellman's method using neostigmine and donepezil as standard inhibitors. Among the screened triazolothiadiazoles, 4j emerged as a lead candidate showing the highest inhibition with an outstanding IC₅₀ value of 0.117 ± 0.007 μM against AChE, which is ∼139-fold greater inhibitory efficacy as compared to neostigmine, whereas 4k displayed ∼506-fold strong inhibition with IC₅₀ of 0.056 ± 0.001 μM against BChE. In the triazolothiadiazine series, 5j and 5e depicted a clear selectivity towards EeAChE with IC₅₀ values of 0.065 ± 0.005 and 0.075 ± 0.001 μM, respectively, which are ∼250- and ∼218-fold stronger inhibition as compared to neostigmine (IC₅₀ = 16.3 ± 1.12 μM). In addition, the synthesized compounds were also tested for their monoamine oxidase (MAO-A and MAO-B) inhibition, where 4a from the triazolothiadiazole series delivered the highest potency against MAO-A with an IC₅₀ value of 0.11 ± 0.005 μM which is ∼33-fold higher inhibition as compared to the standard inhibitor, clorgyline (IC₅₀ = 3.64 ± 0.012 μM), whereas compound 5c from the triazolothiadiazine series turned out to be a lead inhibitor with an IC₅₀ value of 0.011 ± 0.001 μM which is ∼330-fold stronger inhibition. Moreover, compounds 4b (triazolothiadiazole series) and 5o (triazolothiadiazine series) were identified as lead inhibitors against MAO-B. Molecular modelling studies were performed against human AChE and BChE to observe the binding site interactions of these compounds.

$Graphical abstract: Exploration of a library of triazolothiadiazole and triazolothiadiazine compounds as a highly potent and selective family of cholinesterase and monoamine oxidase inhibitors: design, synthesis, X-ray diffraction analysis and molecular docking studies$

This article is part of the themed collection: Molecular modelling

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Exploration of a library of triazolothiadiazole and triazolothiadiazine compounds as a highly potent and selective family of cholinesterase and monoamine oxidase inhibitors: design, synthesis, X-ray diffraction analysis and molecular docking studies†

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Exploration of a library of triazolothiadiazole and triazolothiadiazine compounds as a highly potent and selective family of cholinesterase and monoamine oxidase inhibitors: design, synthesis, X-ray diffraction analysis and molecular docking studies

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