Issue 54, 2015
From the journal:

RSC Advances

Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids†

Romiza R. Mazid,a   Alexandra Cooper,a   Ying Zhang,a   R. Vijayaraghavan,b   Douglas R. MacFarlane,b   Christina Cortez-Jugoc  and  Wenlong Cheng*a  

* Corresponding authors

a Department of Chemical Engineering, Monash University Clayton, Victoria, Australia
E-mail: Wenlong.Cheng@monash.edu

b School of Chemistry, Monash University Clayton, Victoria, Australia

c Monash Institute of Pharmaceutical Sciences, Monash University Parkville, Victoria 3052, Australia

Abstract

Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).

Graphical abstract: Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

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Article information

DOI
https://doi.org/10.1039/C5RA05518K
Article type
Paper
Submitted
28 Mar 2015
Accepted
07 May 2015
First published
07 May 2015

RSC Adv., 2015,5, 43839-43844

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Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

R. R. Mazid, A. Cooper, Y. Zhang, R. Vijayaraghavan, D. R. MacFarlane, C. Cortez-Jugo and W. Cheng, RSC Adv., 2015, 5, 43839 DOI: 10.1039/C5RA05518K

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