An iron–oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase

E. P. Tchesnokov; A. S. Faponle; C. G. Davies; M. G. Quesne; R. Turner; M. Fellner; R. J. Souness; S. M. Wilbanks; S. P. de Visser; G. N. L. Jameson

doi:10.1039/C6CC03904A

An iron–oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase†

E. P. Tchesnokov,^a A. S. Faponle,^b C. G. Davies,^a M. G. Quesne,^b R. Turner,^c M. Fellner,^a R. J. Souness,^a S. M. Wilbanks,^d S. P. de Visser*^b and G. N. L. Jameson*^a

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* Corresponding authors

^a Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand
E-mail: guy.jameson@otago.ac.nz

^b Manchester Institute of Biotechnology and School of Chemical Engineering and Analytical Science, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
E-mail: sam.devisser@manchester.ac.uk

^c Centre for Free Radical Research, University of Otago, 2 Riccarton Ave, PO Box 4345, Christchurch, New Zealand

^d Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand

Abstract

Cysteine dioxygenase is a key enzyme in the breakdown of cysteine, but its mechanism remains controversial. A combination of spectroscopic and computational studies provides the first evidence of a short-lived intermediate in the catalytic cycle. The intermediate decays within 20 ms and has absorption maxima at 500 and 640 nm.

Chemical Communications

An iron–oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase†

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An iron–oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase

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