Original InvestigationsFactor H-related protein-5: A novel component of human glomerular immune deposits*,**,*
Section snippets
Materials and methods
Methods used in this study, identical to those reported in a previous immunohistological study of human renal biopsy specimens,3 are repeated here in summary form. From 100 consecutive percutaneous renal biopsies performed at St Vincent's Hospital Melbourne (Australia), cases were included if there was sufficient material to achieve adequate light microscopic, immunofluorescence, and electron microscopy assessment.
Light and electron microscopy were performed using conventional laboratory
Renal biopsy diagnoses
Ninety-two of 100 consecutive biopsy specimens contained adequate material for both diagnostic purposes and the special immunohistological studies. The final histopathologic diagnoses for these 92 cases are listed in Table 1.
Distribution of FHR-5 by immunofluorescence
There were three patterns of FHR-5 localization, which were similar to patterns observed with other complement proteins.
Discussion
It has long been known that components of the complement pathways are present in glomerular immune deposits.4 Experimental studies have shown a number of glomerular disease models to be complement dependent,5 and the widespread detection of complement in human glomerulonephritis likely is associated with similar complement-dependent mechanisms. Pathogenic roles suggested for complement in immune glomerular injury range from the phlogistic properties of anaphylotoxins to a variety of nonlytic
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2021, Kidney International ReportsCitation Excerpt :FHR-5 staining intensity associated with histologic injury markers, including Oxford E1, S1, and T1/T2 lesions. Before our study, glomerular staining for FHR-5 was observed in patients with membranous nephropathy, lupus nephritis, and diabetic nephropathy,22 which suggested those depositions were not specific to IgAN. However, the glomerular FHR-5 depositions were more abundant in patients with progressive versus stable IgAN.23
The solution structure of the complement deregulator FHR5 reveals a compact dimer and provides new insights into CFHR5 nephropathy
2020, Journal of Biological ChemistryComplement in IgA Nephropathy: The Role of Complement in the Pathogenesis, Diagnosis, and Future Management of IgA Nephropathy
2020, Advances in Chronic Kidney DiseaseCitation Excerpt :Other alternative pathway proteins have also been identified in biopsy specimens of patients with IgAN, including properdin (75-100% of biopsies), FH (30-90%) and two FHR proteins, FHR protein (FHR)1 and FHR5.38,40-43 Glomerular FHR5 deposition was present in all IgAN cases from a series of glomerular diseases and was found in mesangial locations similar to IgA and C3.40 Recently, glomerular FHR5 deposition has been associated with IgAN progression and correlated with deposition of the C3 fragments C3b/iC3b/C3c (the antibody cannot differentiate these fragments) and C3dg as well as C5b9 and negative glomerular FH staining.44
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Supported in part by the National Health and Medical Research Council of Australia and the Baxter Extramural Grant Programme.
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Address reprint requests to Brendan Murphy, MD, PhD, Director of Nephrology, St Vincent's Hospital, Melbourne, Fitzroy 3065, Victoria, Australia. E-mail: [email protected]
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