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Planta Med 2012; 78(08): 787-792
DOI: 10.1055/s-0031-1298431
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum

Jean P. Dzoyem
1   Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Antoine H. L. NKuete
2   Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Victor Kuete
1   Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Michel F. Tala
2   Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Hippolyte K. Wabo
2   Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Santosh K. Guru
3   Cell Signalling Laboratory, Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India
,
Vikrant S. Rajput
4   Clinical Microbiology Unit, Indian Institute of Integrative Medicine, Jammu, India
,
Akash Sharma
4   Clinical Microbiology Unit, Indian Institute of Integrative Medicine, Jammu, India
,
Pierre Tane
2   Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Inshad A. Khan
4   Clinical Microbiology Unit, Indian Institute of Integrative Medicine, Jammu, India
,
Anil K. Saxena
4   Clinical Microbiology Unit, Indian Institute of Integrative Medicine, Jammu, India
,
Hartmut Laatsch
5   Institute of Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany
,
Ning-Hua Tan
6   State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, Peopleʼs Republic of China
› Author Affiliations
Further Information

Publication History

received 14 December 2011
revised 18 March 2012

accepted 20 March 2012

Publication Date:
11 April 2012 (online)

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Abstract

The present study was designed to investigate the antimicrobial activity and the cytotoxicity of the methanol extract (PLA) as well as fractions (PLA1–4) and compounds [cardamomin (1), (±)-polygohomoisoflavanone (2), (S)-(−)-pinostrobin (3), 2′,4′-dihydroxy-3′,6′-dimethoxychalcone (4), (2S)-(−)-5-hydroxy-6,7-dimethoxyflavanone (5), and (2S)-(−)-5,7-dimethoxyflavanone (6)] obtained from leaves of Polygonum limbatum. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against 11 microbial strains including Candida albicans, C. krusei, C. tropicalis, Aspergillus fumigatus, Pseudomonas aeruginosa, Escherichia coli, vancomycin-resistant Enterococcus faecalis (VRE), Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S.epidermidis, and Mycobacterium tuberculosis H37Rv. The sulphorhodamine B cell growth inhibition assay was used to assess the cytotoxicity of the above samples on lung A549 adenocarcinoma, breast carcinoma MCF-7, prostate carcinoma PC-3, cervical carcinoma HeLa, and the acute monocytic leukemia cell line THP-1. The results of the MIC determination indicated that, apart from fraction PLA3, all other fractions as well as PLA and compound 3 were selectively active. MIC values were noted on 100 % of the 11 tested microorganisms for fraction PLA3, 72.7 % for PLA, fraction PLA2, and compound 4, 63.6 % for PLA1, and 54.5 % for fraction PLA4. The results of the cytotoxicity assay revealed that, except for A459 cells, more than 50 % inhibition of the proliferation was obtained with each of the tested samples on at least one of the four other cell lines. IC50 values below 4 µg/mL were obtained with 1 and 4 on THP-1 cells. The overall results of the present study provided baseline information for the possible use of Polygonum limbatum as well as some of the isolated compounds for the control of cancer diseases and mostly leukemia.

Key words

Polygonum limbatum - Polygonaceae - extract - compounds - antimicrobial - cytotoxicity

Supporting Information

 

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