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Thromb Haemost 1968; 20(01/02): 066-077
DOI: 10.1055/s-0038-1651249
Originalarbeiten – Original Articles – Travaux Originaux
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Modification of the Fibrin Agar Plate for Measurements of the Components of the Fibrinolytic System

II. Measurement of Intrinsic Plasminogen Activator and Its Inhibitor (on Type II Fibrin Agar Plate)
P Wolf M. D.
1   External Scientific Staff, Medical Research Council, attached to the Department of Experimental Pathology, University of Birmingham
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Publication History

Publication Date:
27 June 2018 (online)

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Summary

The optimal conditions for plasminogen activation by intrinsic activator (IA) were found on Type II human fibrin agar plates made with .04 M pH 8.5 NH4 acetate buffer.

Intrinsic activation inhibitor (IAI) produced central areas of lysis inhibition.

Methods of quantitating IA and IAI were developed. Activator measurements showed :

1. Undiminished IA concentrations in plasma and serum stored for 3 weeks at + 4° C.

2. 60–80% loss of IA during euglobulin precipitation at pH 5.2 and at pH 6.

3. Presence of a plasminogen activator in commercial thrombin preparation. Inhibitor measurements showed:

1. Decrease of IAI in plasma and serum after 4 days storage at +4° C.

2. A physiological decrease of IAI in late pregnancy.

3. That IAI was composed of either larger or more asymmetrical molecules than either plasminogen or IA.

 

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