CC BY-NC-ND 4.0 · Eur J Dent 2009; 03(02): 100-106
DOI: 10.1055/s-0039-1697415
Original Article
Dental Investigation Society

Increased Levels of 8-Hydroxydeoxyguanosine and Malondialdehyde and its Relationship with Antioxidant Enzymes in Saliva of Periodontitis Patients

Cenk Fatih Canakci
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
,
Yasin Cicek
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
,
Abdulkadir Yildirim
b   Department of Biochemistry, Ataturk University, Faculty of Medicine, Erzurum, Turkey
,
Ufuk Sezer
c   Department of Periodontology, Karadeniz Technical University, Faculty of Dentistry, Trabzon, Turkey
,
Varol Canakci
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
› Author Affiliations
Further Information

Publication History

Publication Date:
27 September 2019 (online)

ABSTRACT

Objectives: The aim of this study was to evaluate 8-hydroxydeoxyguanosine (8-OHdG) and Malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in whole saliva of patients with chronic periodontitis. Moreover, the relationship among the oxidative damage biomarkers, antioxidant enzymes activities and clinical periodontal status were investigated.

Methods: Whole saliva samples were collected from 30 patients with chronic periodontitis and 30 periodontally healthy control. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level, and probing depth were measured. The salivary 8-OHdG level was measured using the ELISA method. SOD and GPx activities and MDA levels were determined spectrophotometrically.

Results: Higher salivary 8-OHdG and MDA levels (P<.001), and lower salivary SOD and GPx activities (P<.05) were detected in periodontitis patients compared to the healthy controls. Additionally, there were significantnegative correlations between salivary levels of 8-OHdG and both salivary SOD and GPx activities as well as between salivary levels of MDA and both salivary SOD and GPx activities (P<.001).

Conclusions: Higher salivary 8-OHdG and MDA levels and lower salivary antioxidant activities seem to reflect increased oxygen radical activity during periodontal inflammation. (Eur J Dent 2009;3:100- 106)

 
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