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Planta Med 2001; 67(2): 103-107
DOI: 10.1055/s-2001-11503
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

A Sesquiterpene Lactone, Costunolide, from Magnolia grandiflora Inhibits NF-κB by Targeting IκB Phosphorylation

Tae Hyeon Koo1, 2 , Jeong-Hyung Lee1 , Yun Joo Park1 , Young-Soo Hong1 , Hang Sub Kim1 , Kyu-Won Kim2 , Jung Joon Lee1,*
  • 1 Anti-Cancer Agent Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea
  • 2 Department of Molecular Biology, Pusan National University, Pusan, Korea
Further Information

Publication History

April 11, 2000

June 24, 2000

Publication Date:
31 December 2001 (online)

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Abstract

A sesquiterpene lactone, costunolide (CTN), was identified from Magnolia grandiflora together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.

Key words

Magnolia grandiflora - Magnoliaceae - costunolide - inducible nitric oxide synthase - NF-κB - IκB

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Dr. Jung Joon Lee

Anti-Cancer Agent Research Laboratory

Korea Research Institute of Bioscience and Biotechnology

P.O. Box 115, Yusong

Taejon 305-600

Korea

Email: jjlee@mail.kribb.re.kr

Fax: +82 42 860 4595

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