Analysis of Several Iridoid and Indole Precursors of Terpenoid Indole Alkaloids with a Single HPLC Run

Denise Dagnino; Jan Schripsema; Robert Verpoorte

doi:10.1055/s-2006-957879

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Planta Med 1996; 62(3): 278-280
DOI: 10.1055/s-2006-957879

Letter

Analysis of Several Iridoid and Indole Precursors of Terpenoid Indole Alkaloids with a Single HPLC Run

Denise Dagnino¹ , Jan Schripsema² , Robert Verpoorte¹

¹Division of Pharmacognosy, Center for Biopharmaceutical Sciences, Leiden University, P.O. Box 9502, NL-2300 RA Leiden, The Netherlands
²Department of Organic Chemistry, The Technical University of Denmark, DK-2800 Lyngby, Denmark

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Publication History

1995

1995

Publication Date:
04 January 2007 (online)

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Abstract

An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 µm, 250 × 4 mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.

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