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DOI: 10.1055/s-0028-1084534
Determination of furanocoumarins in Citrus hystrix by HPLC photodiode array
A rapid and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method was used for determination of furanocoumarins in methanolic extracts of the peel of Citrus hystrix DC. HPLC separation was performed on a C-12 Phenomenex column (Synergi Max-RP, 150×4.6mm, 4µm particle size) used as a stationary phase and acetonitrile–water gradient as a mobile phase. Flow-rate, detection wavelength and temperature were adjusted to 1.0 mL/min, 254nm and 40 oC, respectively. Calibration plots were linear in a range of 31.74–241µg/mL of oxypeucedanin hydrate and 32.79–249µg/mL of 6', 7'-dihydrobergamottin with a good linearity (r2 =1), precision (intra-day R.S.D. <0.77%, inter-day R.S.D. <3.09%) and recovery rates of 96.83 to 101.23%. Limit of detection (LOD) was found to be 0.31µg/mL for both compounds. The analysis of C. hystrix peel samples from 10 different locations of Thailand revealed that 6', 7'-dihydrobergamottin (0.38–0.88%w/w) was dominant in all specimens, followed by oxypeucedanin hydrate (0.32–0.54%w/w).
Acknowledgements: This work was granted by The Thailand Research Fund (The Royal Golden Jubilee Ph.D. Program, 5. PN.MU/47/H.1) and Institut für Pharmazie, Abteilung Pharmakognosie, Universität Innsbruck