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DOI: 10.1055/s-0032-1321210
Leonurus cardiaca, L. Japonicus, Leonotis leonurus: Quantitative HPLC and instrumental HPTLC determination of fourteen phenolics
Leonurus cardiaca, Leonurus japonicus, and Leonotis leonurus are traditionally used for cardiovascular diseases in Europe, East Asia, and Africa. Still, only a single HPLC analytical study on potentially bioactive phenolics, solely for L. cardiaca, has been reported. Here, a novel RP-HPLC method is presented for quantification of 12 phenolics (chlorogenic, caffeic, ferulic, rosmarinic, cichoric acid, lavandulifolioside, verbascoside, hyperoside, isoquercitrin, rutoside, apigenin-7-O-D-glucoside, quercitrin) in 18 herbal and seed samples of the 3 species as well as in a L. cardiaca refined extract [1]. The theorized presence of leonoside A and B was refuted via HPTLC. Only ferulic acid was found in every sample, whereas rosmarinic acid and apigenin-7-O-D-glucoside were not detected in any sample. Chlorogenic, caffeic, cichoric acid and rutoside were detected in all 3 species. Lavandulifolioside and verbascoside were not present in any sample of L. japonicus, but in every sample of the aerial parts of L. cardiaca. Lavandulifolioside was found in this first ever HPLC analysis on phenolics of L. leonurus. Hyperoside was not found in L. cardiaca but in both L. japoni- cus and L. leonurus, whereas isoquercitrin was detected in L. cardiaca and L. leonurus but not in L. japonicus. This approach facilitates identification and quality control via HPLC/HPTLC fingerprints.