Semin Reprod Med 2014; 32(04): 272-282
DOI: 10.1055/s-0034-1375179
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Laboratory Procedures for Human In Vitro Fertilization

Basak Balaban
1   Assisted Reproduction Unit, VKF American Hospital of Istanbul, Istanbul, Turkey
,
Denny Sakkas
2   Boston IVF, Waltham, Massachusetts
,
David K. Gardner
3   Department of Zoology, University of Melbourne, Melbourne Australia
› Author Affiliations
Further Information

Publication History

Publication Date:
11 June 2014 (online)

Abstract

Successful and consistent outcomes in human in vitro fertilization (IVF) can be readily achieved by all IVF clinics through consideration and optimization of each procedure associated with the collection and processing of gametes, culminating in the resultant culture and transfer of healthy embryos. Furthermore, understanding the interactions between the individual components of the IVF cycle will assist when trouble-shooting possible problems in a laboratory which could have an adverse effect on cycle outcome. This article will review handling of oocytes and embryo culture, preparation of gametes for insemination and microinjection, selection of the most viable gametes and embryos, cryopreservation, and successful embryo transfer from the laboratory perspective.

 
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