Planta Med 2018; 84(04): 242-249
DOI: 10.1055/s-0043-123074
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Extraction Method and Analysis of Cannabinoids in Cannabis Olive Oil Preparations

Antonella Casiraghi
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Gabriella Roda
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Eleonora Casagni
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Cecilia Cristina
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Umberto Maria Musazzi
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Silvia Franzè
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Paolo Rocco
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Claudia Giuliani
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Gelsomina Fico
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Paola Minghetti
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
,
Veniero Gambaro
Dept. Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy
› Author Affiliations
Further Information

Publication History

received 15 March 2017
revised 31 October 2017

accepted 03 November 2017

Publication Date:
04 December 2017 (online)

Abstract

Recently, an increasing number of pharmacists had to supply medicinal products based on Cannabis sativa L. (Cannabaceae), prescribed by physicians to individual patients. Cannabis olive oil preparation is the first choice as a concentrated extract of cannabinoids, even though standardized operative conditions for obtaining it are still not available. In this work, the impact of temperature and extraction time on the concentration of active principles was studied to harmonize the different compounding methods, optimize the extraction process, and reduce the variability among preparations. Moreover, starting from the cannabis inflorescence, the effect of temperature on tetrahydrocannabinolic acid decarboxylation was evaluated. For the analysis, a GC/MS method, as suggested by the Italian Ministry of Health, and a GC/flame ionization detection method were developed, validated, and compared.

Supporting Information

 
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