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Planta Med 1988; 54(5): 393-397
DOI: 10.1055/s-2006-962479
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© Georg Thieme Verlag Stuttgart · New York

Accumulation of Indole Alkaloids in a Suspension Culture of Tabernaemontana divaricata 1

R. van der Heijden2 , A. Hermans-Lokkerbol2 , L. P. J. de Kool2 , P. J. Lamping2 , P. A. A. Harkes3 , R. Verpoorte2
  • 2Biotechnology Delft Leiden (BDL), Center for Bio-Pharmaceutical Sciences, Division of Pharmacognosy, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
  • 3Department of Plant Molecular Biology, Subfaculty of Biology, Leiden University, Nonnensteeg 3, 2311 VJ Leiden, The Netherlands.
1 Part 19 in the series “Pharmacognostical Studies of Tabernaemontana species”. For part 18 see Ref. (1).
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Publication History

1988

Publication Date:
24 January 2007 (online)

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Abstract

Cell suspension cultures of Tabernaemontana divaricata were found to produce relatively large amounts of indole alkaloids. For their isolation an ion-pair DCCC method was used in combination with preparative TLC. The alkaloids were identified as tabernaemontanine, perivine, vobasine, voaphylline, voaphylline-hydroxyindolenine, vallesamine, apparicine, 16-hydroxy-16,22-dihydroapparicine, pericyclivine, tubotaiwine, 19-S-heyneanine, and coronaridine. Voaphylline, the main alkaloid, was produced during growth and early stationary phase and reached a maximum of 23 mg/l at day 19 of the growth cycle. After this maximum voaphylline was rapidly metabolized. Apparicine, vobasine, and coronaridine reached their maximum levels at a later stage of the growth cycle. Tubotaiwine accumulation showed a similar profile as that of voaphylline. In light-grown cells the total production was about 2 times higher than in dark-grown cells, with respective main products voaphylline and apparicine.

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