Abstract
The genetic variation of Ascochyta lentis in Australia was investigated using random amplified polymorphic DNA (RAPD) analysis of 23 isolates. Of the 87 loci assessed, 86 were polymorphic. The most closely related isolates were from Victoria and South Australia, and they shared identical banding profiles. The most genetically distant isolates were from South Australiaand Western Australia (83% dissimilar). Analysis of molecular variance showed low genetic variation between groups of isolates originating from either within or outside Australia (4%). Large variation within each group (96%) indicated similar pathogen variability within and outside Australia. Partitioning of variation within Australian states was shown. Isolates collected within South Australia were least variable (1%) and isolates collected within Western Australia were most variable (61%). Increased variation in Western Australia is thought to have occurred through multiple introductions andlor the frequent presence of both mating types. Furthermore, two RAPD markers used in unison were able to explaino ver 50% of the variation in isolate pathogenicity. No RAPD markers were found that discriminated mating type.
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Ford, R., Garnier-Géré, P.H., Nasir, M. et al. Structure of Ascochyta lentis in Australia revealed with random amplified polymorphic DNA (RAPD) markers. Australasian Plant Pathology 29, 36–45 (2000). https://doi.org/10.1071/AP00006
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DOI: https://doi.org/10.1071/AP00006