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Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii ) for disease trait dissection in perennial ryegrass (Lolium perenne L.)

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Abstract

Genetic analysis of host resistance to the crown rust pathogen of ryegrasses has traditionally involved the use of composite urediniospore collections, which may contain multiple genotypes and virulence races. Consequently, discrimination between major gene responses to alternative races and quantitative resistance has proved challenging for disease trait-dissection studies.Amethod of propagation on detached leaves has been used to derive genetically homogenised crown rust pathogen lines from bulked spore collections obtained from five distinct Australian locations. Spores from individual pustules were repeatedly reinoculated onto fresh leaves. Amplicon complexity was assessed at the conclusion of each cycle using crown rust pathogen expressed sequence tag (EST)-derived simple sequence repeat (SSR) primer pairs. The mean number of amplification products per isolate declined over successive rounds of reinfection. The number of rounds of sequential infection required for decay to stable levels was dependent on the diversity present within the bulked spore collection. Large-scale propagation of the putative homogenised populations developed in the present study may provide inoculum for infection studies to allow quantitative trait loci mapping of specific gene-for-gene responses. The methodology has significant applications for the pyramiding of resistance genes specific to diverse crown rust pathogen genotypes during cultivar development and for pathotyping of existing elite germplasm. This approach is also applicable to other Puccinia species.

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References

  • Aldaoud R, Anderson MW, Reed KFM, Smith KF (2004) Evidence of pathotypes among Australian isolates of crown rust infecting perennial ryegrass. Plant Breeding 123, 395–397. doi: 10.1111/j.1439-0523. 2004.00951.x

    Article  Google Scholar 

  • Bradley SG (1962) Parasexual phenomena in microorganisms. Annual Review of Microbiology 16, 35–52. doi: 10.1146/annurev.mi.16. 100162.000343

    Article  CAS  PubMed  Google Scholar 

  • Brake VM, Irwin JAG, Park RF (2001) Genetic variability in Australian isolates of Puccinia coronata f. sp. avenae assessed with molecular and pathogenicity markers. Australasian Plant Pathology 30, 259–266. doi: 10.1071/AP01039

    Article  Google Scholar 

  • Brown JKM, Hovmøller MS (2002) Aerial dispersal of pathogens on the global and continental scales and its impact on plant disease. Science 297, 537–541. doi: 10.1126/science.1072678

    Article  CAS  PubMed  Google Scholar 

  • Cornish MA, Hayward MD, Lawrence MJ (1979) Self-incompatibility in ryegrass. I. Genetic control in diploid Lolium perenne L. Heredity 43, 95–106. doi: 10.1038/hdy.1979.63

    Article  Google Scholar 

  • Dean FB, Hosono S, Fang L, Wu X, Faruqi F, et al. (2002) Comprehensive human genome amplification using multiple displacement amplification. Proceedings of the National Academy of Sciences of the United States of America 99, 5261–5266. doi: 10.1073/pnas. 082089499

    Article  CAS  PubMed  Google Scholar 

  • Dracatos PM, Dumsday JL, Olle RS, Cogan NOI, Dobrowolski MP, Fujimori M, Roderick H, Stewart AV, Smith KF, Forster JW (2006) Development and characterisation of EST-SSR markers from the crown rust pathogen of ryegrass (Puccinia coronata Corda f. sp. lolii Brown). Genome 49, 572–583. doi: 10.1139/G06-006

    Article  CAS  PubMed  Google Scholar 

  • Dracatos P, Dumsday J, Stewart A, Dobrowolski M, Cogan N, Smith K, Forster JW (2008) Genetic diversity in Australasian populations of the crown rust pathogen of ryegrasses (Puccinia coronata f. sp. lolii). In ‘Molecular Breeding of Forage and Turf: the Proceedings of the 5th International Symposium on the Molecular Breeding of Forage and Turf’. (Eds T Yamada, G Spangenberg) pp. 275–285. (Springer: New York)

    Google Scholar 

  • Dumsday JL, Smith KF, Forster JW, Jones ES (2003) SSR-based genetic linkage analysis of resistance to crown rust (‘Puccinia coronata f. sp. lolii) in perennial ryegrass (Lolium perenne). Plant Pathology 52, 628–637. doi: 10.1046/j.1365-3059.2003.00884.x

    Article  Google Scholar 

  • Hovmøller MS, Justesen AF, Brown JKM (2002) Clonality and longdistance migration of Puccinia striiformis f. sp. tritici in north-west Europe. Plant Pathology 51, 24–32. doi: 10.1046/j.1365-3059.2002. 00652.x

    Article  Google Scholar 

  • Keiper FJ, Hayden MJ, Park RF, Wellings CR (2003) Molecular genetic variability of Australian isolates of five cereal rust pathogens. Mycological Research 107, 545–556. doi: 10.1017/ S0953756203007809

    Article  CAS  PubMed  Google Scholar 

  • Keiper FJ, Haque MS, Hayden MJ, Park RF (2006) Genetic diversity in Australian populations of Puccinia graminis f. sp. avenae. Phytopathology 96, 96–104. doi: 10.1094/PHYTO-96-0096

    Article  CAS  PubMed  Google Scholar 

  • Kimbeng CA (1999) Genetic basis of crown rust resistance in perennial ryegrass, breeding strategies, and genetic variation among pathogen populations: a review. Australian Journal of Experimental Agriculture 39, 361–378. doi: 10.1071/EA98111

    Article  Google Scholar 

  • McVeigh KJ (1975) Breeding for resistance to crown rust (Puccinia coronata Corda var. lolii Brown) in turf type perennial ryegrass (Lolium perenne). Ph.D. Thesis, Rutgers University, New Brunswick, NJ, USA.

    Google Scholar 

  • Muylle H, Baert J, Van Bockstaele E, Pertijs J, Roldan-Ruiz I (2005) Four QTLs determine crown rust (Puccinia coronata f. sp. Lolii) resistance in a perennial ryegrass (Lolium perenne) population. Heredity 95, 348–357. doi: 10.1038/sj.hdy.6800729

    Article  CAS  PubMed  Google Scholar 

  • Nagarajan S, Singh DV (1990) Long-distance dispersion of rust pathogens. Annual Review of Phytopathology 28, 139–153. doi: 10.1146/annurev. py.28.090190.001035

    Article  CAS  PubMed  Google Scholar 

  • Park RF (1996) Pathogenic specialisation of Puccinia recondite f. sp. tritici in Australia and New Zealand in 1990 and 1991. Australasian Plant Pathology 25, 12–17. doi: 10.1071/AP96003

    Article  Google Scholar 

  • Park RF, Jahoor A, Felsenstein FG (2000) Population structure of Puccinia recondite in Western Europe during 1995, as assessed by variability in pathogenicity and molecular markers. Phytopathology 148, 169–179. doi: 10.1046/j.1439-0434.2000.00458.x

    Article  CAS  Google Scholar 

  • Potter LR, Cagas B, Paul VH, Birckenstaedt E (1990) Pathogenicity of some European collections of crown rust (Puccinia coronata Corda) on cultivars of perennial ryegrass. Phytopathology 130, 119–126. doi: 10.1111/j.1439-0434.1990.tb01159.x

    Article  Google Scholar 

  • Price T (1987) Ryegrass rust in Victoria. Plant Protection Quarterly 2, 189.

    Google Scholar 

  • Schejbel B, Jensen LB, Xing Y, Lübberstedt T (2007) QTL analysis of crown rust resistance in perennial ryegrass under conditions of natural and artificial infection. Plant Breeding 126, 347–352. doi: 10.1111/j.1439-0523.2007.01385.x

    Article  Google Scholar 

  • Simons MD (1970) ‘Crown rust of oats and grasses.’ Monograph no. 5. (The Heffernan Press: Worcester, MA)

    Google Scholar 

  • Skipp RA, White DWR (1988) Somaclonal variation in resistance of perennial ryegrass to crown rust. Proceedings of the New Zealand Grasslands Association 49, 93–96.

    Google Scholar 

  • Studer B, Boller B, Bauer E, Posselt U, Widmer F, Kölliker R (2007) Consistent detection of QTLs for crown rust resistance in Italian ryegrass (Lolium multiflorum Lam.) across environments and phenotyping methods. Theoretical and Applied Genetics 115, 9–17. doi: 10.1007/s00122-007-0535-z

    Article  PubMed  Google Scholar 

  • Takahashi W, Fujimori M, Miura Y, Komatsu T, Nishizawa Y, Hibi T, Takamizo T (2005) Increased resistance to crown rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene. Plant Cell Reports 23, 811–818. doi: 10.1007/ s00299-004-0900-1

    Article  CAS  PubMed  Google Scholar 

  • Thorogood D, Paget M, Humphreys M, Turner L, Armstead I, Roderick H (2001) QTL analysis of crown rust resistance in perennial ryegrass — implications for breeding. International Turfgrass Society Research Journal 9, 218–223.

    Google Scholar 

  • Wilkins PW (1975) Inheritance of resistance to Puccinia coronata Corda and Rhynchosporium orthosporium Caldwell in Italian ryegrass. Euphytica 24, 191–196. doi: 10.1007/BF00147185

    Article  Google Scholar 

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Correspondence to J. W. Forster.

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Dracatos, P.M., Dobrowolski, M.P., Lamb, J. et al. Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii ) for disease trait dissection in perennial ryegrass (Lolium perenne L.). Australasian Plant Pathology 38, 55–62 (2009). https://doi.org/10.1071/AP08077

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