Abstract
A sequential batch testing procedure was used in conjunction with ELISA or cDNA probes to estimate the incidence of chrysanthemum stunt viroid (CSVd), chrysanthemum carlavirus B (CVB), tomato aspermy cucumovirus (TAV) and tomato spotted wilt tospovirus (TSWV) in chrysanthemum (Dendranthema grandiflora) crops in Australia. A total of 2480 samples of potted, field-grown and greenhouse-grown chrysanthemums was tested from six Australian States. All four of these pathogens were detected. CVB (39.2%) and TAV (6.7%) were the most prevalent, CSVd (0.7%) was detected only in greenhouse chrysanthemums from Queensland, and TSWV (1.4%) was detected only in field-grown chrysanthemums from Western Australia.
Similar content being viewed by others
References
Bitterlich, I. and MacDonald, L.S. (1993) — The prevalence of tomato spotted wilt virus in weeds and crops in south-westem British Columbia. Canadian Plant Disease Survey 73: 137–143.
Clark, M.F. and Adams, A.N. (1977) — Characteristics of the microplate method of enzyme-linked immuno-sorbent assay for the detection of plant viruses. Journal of General Virology 34: 475–83.
German, T.L., Ullman, D.E. and Moyer, J.W. (1992) — Tospoviruses: Diagnosis, molecular biology, phylogeny and vector relationships. Annual Review of Phytopathology 30: 315–348.
Hausbeck, M.K., Welliver, R.A., Derr, M.A. and Gildow, F.E. (1992) — Tomato spotted wilt virus survey among greenhouse ornamentals in Pennsylvania. Plant Disease 76: 795–800.
Hepworth, G. (1992) — Confidence intervals for proportions estimated by group testing. Proceedings of the XVIth International Biometric Conference, Hamilton, New Zealand.
Hollings, M. (1955) — Investigation of chrysanthemum viruses. 1. Aspermy flower distortion. Annals of Applied Biology 43: 86–102.
Hollings, M. (1957) — Investigation of chrysanthemum viruses. II. Virus B (mild mosaic) and chrysanthemum latent virus. Annals of Applied Biology 45: 589–602.
Malipatil, M.B., Postle, A.C., Osmelak, J.A., Hill, M.F. and Moran, J.R. (1993) — First record of Frankhiella occidentalis (Pergande) in Australia (Thysanoptera: Thripidea). Journal of the Australian Entomological Society 32: 378.
Moran. J.R. and Bate, C.E. (1988) — Chrysanthemum stunt viroid. The Flower Link June 1988, pp. 15–19.
Moran, J.R. and Curtis, C. (1986) — Virus diseases of chrysanthemums. Department of Agriculture, Victoria, Agnote, Agdex 282/633.
Rodoni, B.C., Hepworth, G., Richardson, C. and Moran, J.R. (1994) — The use of a sequential batch testing procedure and ELISA to determine the incidence of five viruses in Victorian cut-flower Sim carnations. Australian Journal of Agricultural Research 45: 223–230.
Walter, S.D., Hildreth, S.W. and Beaty, B.J. (1980) — Estimation of infection rates in populations of organisms using pools of variable size. American Journal of Epidemiology 112: 124–128.
Wilson, J.M. and Moran, J.R. (1983) — The incidence of chrysanthemum virus B, tomato aspermy virus and tomato spotted wilt virus in commercial chrysanthemum crops in Victoria. Australasian Plant Pathology 12: 17.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hill, M.F., Giles, R.J., Moran, J.R. et al. The incidence of chrysanthemum stunt viroid, chrysanthemum B carlavirus, tomato aspermy cucumovirus and tomato spotted wilt tospovirus in Australian chrysanthemum crops. Australasian Plant Pathology 25, 174–178 (1996). https://doi.org/10.1071/AP96030
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1071/AP96030