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Contents Vol 65(5)

Copper Modulation of Amyloid Beta 42 Interactions with Model Membranes

Daniel K. Weber A , John D. Gehman A , Frances Separovic A and Marc-Antoine Sani A B
+ Author Affiliations
- Author Affiliations

A School of Chemistry, Bio21 Institute, University of Melbourne, Melbourne, Vic. 3010, Australia.

B Corresponding author. Email: msani@unimelb.edu.au

Australian Journal of Chemistry 65(5) 472-479 https://doi.org/10.1071/CH12015
Submitted: 15 January 2012  Accepted: 9 February 2012   Published: 27 March 2012

Abstract

Growing evidence supports that interactions of the amyloid-β peptide Aβ(1–42) with neuronal cell membranes and copper are involved in Alzheimer’s disease pathogenesis. We report using solid-state NMR that the peptide significantly perturbed the phosphate and upper acyl chain region of bilayers comprising brain total lipid extract to cause domain segregation. Deep headgroup perturbations were also realized for palmitoyloleoylphospatidylcholine–cholesterol model systems; however, incorporating 10 % palmitoyloleoylphosphatidylserine or the ganglioside GM1 resulted in a more peripheral interaction. Cu2+ at a 1 : 7 molar ratio to peptide caused deeper penetration into model systems, but partially attenuated interactions with brain total lipid extract. Thioflavin T assay showed that bilayers affected amyloid formation in a mode dependant on lipid content, and was further modulated by addition of Cu2+. Our data support that ternary interactions between Cu2+, lipids and Aβ(1–42) may have significance in Alzheimer’s disease, and challenge the validity of model bilayers as substitutes for natural systems.


References

[1]  G. G. Glenner, C. W. Wong, Biochem. Biophys. Res. Commun. 1984, 120, 885.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXitVOntLw%3D&md5=bd5616ed1afe98575415822739f4b1b6CAS |

[2]  C. J. Pike, A. J. Walencewicz, C. G. Glabe, C. W. Cotman, Eur. J. Pharmacol. (Mol. Pharmacol. Sect.) 1991, 207, 367.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXlvVSksrY%3D&md5=53791b09976e68d2692883066846cefeCAS |

[3]  D. Burdick, B. Soreghan, M. Kwon, J. Kosmoski, M. Knauer, A. Henschen, J. Yates, C. Cotman, C. Glabe, J. Biol. Chem. 1992, 267, 546.
         | 1:CAS:528:DyaK38XjvFSmtA%3D%3D&md5=de8621e001fde5000109ef8d722f76d5CAS |

[4]  Y. Zhang, R. McLaughlin, C. Goodyer, A. LeBlanc, J. Cell Biol. 2002, 156, 519.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhtVOqtLw%3D&md5=8bca4a6876d9aef1bddefe5913c0e990CAS |

[5]  A. T. Petkova, Y. Ishii, J. J. Balbach, O. N. Antzutkin, R. D. Leapman, F. Delaglio, R. Tycko, Proc. Natl. Acad. Sci. USA 2002, 99, 16742.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhvV2iug%3D%3D&md5=ead7303cbe37478da0a760395c5f2bedCAS |

[6]  T. Lührs, C. Ritter, M. Adrian, D. Riek-Loher, B. Bohrmann, H. Döbeli, D. Schubert, R. Riek, Proc. Natl. Acad. Sci. USA 2005, 102, 17342.
         | Crossref | GoogleScholarGoogle Scholar |

[7]  D. M. Walsh, I. Klyubin, J. V. Fadeeva, W. K. Cullen, R. Anwyl, M. S. Wolfe, M. J. Rowan, D. J. Selkoe, Nature 2002, 416, 535.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XivVOntL8%3D&md5=29fa886fa8cf52577fcbafdbebbab4ffCAS |

[8]  B. DaRocha-Souto, T. C. Scotton, M. Coma, A. Serrano-Pozo, T. Hashimoto, L. Serenó, M. Rodríguez, B. Sánchez, B. T. Hyman, T. Gómez-Isla, J. Neuropathol. Exp. Neurol. 2011, 70, 360.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXkvF2isb0%3D&md5=b12ccf0eaa01a022b2d1000a5aceb508CAS |

[9]  N. Demeester, G. Baier, C. Enzinger, M. Goethals, J. Vandekerckhove, M. Rosseneu, C. Labeur, Mol. Membr. Biol. 2000, 17, 219.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXis1yhsr4%3D&md5=971e0a55a1e3dba25f2e37b80849b24cCAS |

[10]  M. Kawahara, I. Ohtsuka, S. Yokoyama, M. Kato-Negishi, Y. Sadakane, Int. J. Alzheimer’s Dis. 2011, Apr 12; 2011:304583.

[11]  R. Kayed, Y. Sokolov, B. Edmonds, T. M. McIntire, S. C. Milton, J. E. Hall, C. G. Glabe, J. Biol. Chem. 2004, 279, 46363.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXptFaqtr8%3D&md5=53cb3b6ccfda862020719f4ede190bc6CAS |

[12]  N. Arispe, H. B. Pollard, E. Rojas, Proc. Natl. Acad. Sci. USA 1993, 90, 10573.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXnsVOisQ%3D%3D&md5=303c0935374d9de8a1bf82c729cdb5fcCAS |

[13]  H. Lin, R. Bhatia, R. Lal, FASEB J. 2001, 15, 2433.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXoslSisrs%3D&md5=746d621152bbbab2bc91788d323f2850CAS |

[14]  A. Quist, I. Doudevski, H. Lin, R. Azimova, D. Ng, B. Frangione, B. Kagan, J. Ghiso, R. Lal, Proc. Natl. Acad. Sci. USA 2005, 102, 10427.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntVSit7w%3D&md5=3cef39fbd432cb1089b62a71c6bda599CAS |

[15]  E. E. Ambroggio, D. H. Kim, F. Separovic, C. J. Barrow, K. J. Barnham, L. A. Bagatolli, G. D. Fidelio, Biophys. J. 2005, 88, 2706.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjtFCjtr0%3D&md5=36c8a4d93321711e3f56f4a11604c6baCAS |

[16]  T. L. Williams, I. J. Day, L. C. Serpell, Langmuir 2010, 26, 17260.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1Cmtb7J&md5=1ad42b720492889c03392f4e4a6b8d4fCAS |

[17]  T. L. Williams, B. R. G. Johnson, B. Urbanc, A. T. A. Jenkins, S. D. A. Connell, L. C. Serpell, Biochem. J. 2011, 439, 67.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFKitb7J&md5=8f82d009d572ff280afcb6489b1ce3beCAS |

[18]  D. P. Smith, D. G. Smith, C. C. Curtain, J. F. Boas, J. R. Pilbrow, G. D. Ciccotosto, T. L. Lau, D. J. Tew, K. Perez, J. D. Wade, A. I. Bush, S. C. Drew, F. Separovic, C. L. Masters, R. Cappai, K. J. Barnham, J. Biol. Chem. 2006, 281, 15145.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XltVequrs%3D&md5=72382ee11f8089a38d609f9193bcf696CAS |

[19]  M. A. Lovell, J. D. Robertson, W. J. Teesdale, J. L. Campbell, W. R. Markesbery, J. Neurol. Sci. 1998, 158, 47.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsVentbo%3D&md5=d74d09d0be035a0f90d20099acbd1210CAS |

[20]  K. J. Barnham, F. Haeffner, G. D. Ciccotosto, C. C. Curtain, D. Tew, C. Mavros, K. Beyreuther, D. Carrington, C. L. Masters, R. A. Cherny, R. Cappai, A. I. Bush, FASEB J. 2004, 18, 1427.
         | 1:CAS:528:DC%2BD2cXntlKlu7g%3D&md5=67d7e783b24391addc2b3af15a61ed3eCAS |

[21]  K. J. Barnham, G. D. Ciccotosto, A. K. Tickler, F. E. Ali, D. G. Smith, N. A. Williamson, Y. H. Lam, D. Carrington, D. Tew, G. Kocak, I. Volitakis, F. Separovic, C. J. Barrow, J. D. Wade, C. L. Masters, R. A. Cherny, C. C. Curtain, A. I. Bush, R. Cappai, J. Biol. Chem. 2003, 278, 42959.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXosFSkt7g%3D&md5=032067d71e1e5a73757ba2f8ebbee4e7CAS |

[22]  F. E. Ali, K. J. Barnham, C. J. Barrow, F. Separovic, J. Inorg. Biochem. 2004, 98, 173.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpsVSqtbo%3D&md5=9d95642009fb33218f42b00f07e7bb3fCAS |

[23]  D. A. Butterfield, C. M. Lauderback, Free Radic. Biol. Med. 2002, 32, 1050.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjvVehur0%3D&md5=28c0d3875c3f72d7ad6be7cf51ca5618CAS |

[24]  V. Martín, N. Fabelo, G. Santpere, B. Puig, R. Marín, I. Ferrer, M. Díaz, J. Alzheimers Dis. 2010, 19, 489.

[25]  T. L. Lau, J. D. Gehman, J. D. Wade, C. L. Masters, K. J. Barnham, F. Separovic, BBA-Biomembranes 2007, 1768, 3135.
         | 1:CAS:528:DC%2BD2sXhsVCksbfJ&md5=db873fca34765290143d48709be2bf3eCAS |

[26]  T. L. Lau, E. E. Ambroggio, D. J. Tew, R. Cappai, C. L. Masters, G. D. Fidelio, K. J. Barnham, F. Separovic, J. Mol. Biol. 2006, 356, 759.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XotVKqtw%3D%3D&md5=7c5995b37dbe0530066db146366df413CAS |

[27]  T. L. Lau, J. D. Gehman, J. D. Wade, K. Perez, C. L. Masters, K. J. Barnham, F. Separovic, BBA-Biomembranes 2007, 1768, 2400.
         | 1:CAS:528:DC%2BD2sXhtFGrsr3N&md5=6cedcb3b7555c6d120a211cea792ced0CAS |

[28]  J. D. Gehman, C. C. O’Brien, F. Shabanpoor, J. D. Wade, F. Separovic, Eur. Biophys. J. 2008, 37, 333.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXitlOhurk%3D&md5=f925dd90d09d3ba38a9629e42035623aCAS |

[29]  M. P. Mattson, J. G. Begley, R. J. Mark, K. Furukawa, Brain Res. 1997, 771, 147.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmtFKgsbg%3D&md5=1a1b923921ded35da5317cd77d4d8c92CAS |

[30]  G. W. Stockton, I. C. P. Smith, Chem. Phys. Lipids 1976, 17, 251.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXislSrtg%3D%3D&md5=8f38ac13e73a44bf42c925406b015c25CAS |

[31]  C. C. Curtain, F. E. Ali, D. G. Smith, A. I. Bush, C. L. Masters, K. J. Barnham, J. Biol. Chem. 2003, 278, 2977.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXmt1Gmuw%3D%3D&md5=d097e01e0eb6e15368b2afed113eb419CAS |

[32]  S. Dante, T. Hauß, N. A. Dencher, Eur. Biophys. J. 2006, 35, 523.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xms1GqtLw%3D&md5=adeeeac5b0ffcd482fbb0318de1ea98dCAS |

[33]  L. P. Choo-Smith, W. Garzon-Rodriguez, C. G. Glabe, W. K. Surewicz, J. Biol. Chem. 1997, 272, 22987.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmt1WjtL0%3D&md5=3d034377ee6a44461e1261b347041d29CAS |

[34]  J. McLaurin, A. Chakrabartty, Eur. J. Biochem. 1997, 245, 355.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXivFCgsb8%3D&md5=eccb248aff580044625404a23385048fCAS |

[35]  G. D. Ciccotosto, D. J. Tew, S. C. Drew, D. G. Smith, T. Johanssen, V. Lal, T. L. Lau, K. Perez, C. C. Curtain, J. D. Wade, F. Separovic, C. L. Masters, J. P. Smith, K. J. Barnham, R. Cappai, Neurobiol. Aging 2011, 32, 235.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFamtrnM&md5=4e8b8f92c33e95574a562ed16939479fCAS |

[36]  K. Yanagisawa, A. Odaka, N. Suzuki, Y. Ihara, Nat. Med. 1995, 1, 1062.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXosVGgu7c%3D&md5=7fba491bb42431d8b42bcd916a4f0071CAS |

[37]  E. Y. Chi, S. L. Frey, K. Y. C. Lee, Biochemistry 2007, 46, 1913.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXoslKksA%3D%3D&md5=9fabbcbf726038da8be3928e2e49e091CAS |

[38]  M. A. Sani, J. D. Gehman, F. Separovic, FEBS Lett. 2011, 585, 749.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjsF2jurk%3D&md5=5e6c55950c341326d097c98cb1d9b3e7CAS |

[39]  V. N. Uversky, J. Li, A. L. Fink, J. Biol. Chem. 2001, 276, 10737.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjvFCqtLs%3D&md5=76c581eb5b14974d01e8db3c98436e53CAS |

[40]  F. J. Richards, J. Exp. Bot. 1959, 10, 290.
         | Crossref | GoogleScholarGoogle Scholar |

[41]  J. P. Greenfield, J. Tsai, G. K. Gouras, B. Hai, G. Thinakaran, F. Checler, S. S. Sisodia, P. Greengard, H. Xu, P. Natl. Acad. Sci. USA 1999, 96, 742.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXmtlCqsA%3D%3D&md5=082e215250c3280351263560d9935b7cCAS |

[42]  F. Delaglio, S. Grzesiek, G. W. Vuister, G. Zhu, J. Pfeifer, A. Bax, J. Biomol. NMR 1995, 6, 277.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXhtVSmurfK&md5=dd6f47632e7b9b143d1d17a1e9daf36eCAS |

[43]  G. E. Pake, J. Chem. Phys. 1948, 16, 327.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaH1cXhvV2qsw%3D%3D&md5=b7a5ae8817d49b4f1edd819f1b7870b9CAS |

[44]  M. A. McCabe, S. R. Wassall, Solid State Nucl. Magn. Reson. 1997, 10, 53.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXnvFGrurs%3D&md5=9d06c63adcff73f9a03e5a42a0f7ec80CAS |

[45]  K. P. Whittall, E. Sternin, M. Bloom, A. L. Mackay, J. Magn. Reson. 1989, 84, 64.
         | Crossref | GoogleScholarGoogle Scholar |

[46]  M. Rance, R. A. Byrd, J. Magn. Reson. 1983, 52, 221.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXhvFSnsbo%3D&md5=35d186a3b61fcba2a5ad4667e2095b07CAS |

[47]  M. Galassi, J. Davies, J. Theiler, B. Gough, G. Jungman, P. Alken, M. Booth, F. Rossi, GNU Scientific Library Reference Manual 2011, revised 3rd edition (Free Software Foundation: Boston, MA). Available at: http://www.gnu.org/software/gsl

[48]  D. I. Fernandez, M. A. Sani, J. D. Gehman, K. S. Hahm, F. Separovic, Eur. Biophys. J. 2011, 40, 471.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXkt1Wqu7Y%3D&md5=0456b137e327e9188761bcdd00ab0415CAS |

[49]  D. I. Fernandez, M. A. Sani, F. Separovic, Aust. J. Chem. 2011, 64, 798.
         | 1:CAS:528:DC%2BC3MXotFyjsrw%3D&md5=8375cb81bc701fef53ea91529937a838CAS |

[50]  F. Separovic, B. Cornell, R. Pace, Chem. Phys. Lipids 2000, 107, 159.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXot12rtL8%3D&md5=d76879b3afabdea9cdfa1119f2b4bf2cCAS |

[51]  P. Hortschansky, V. Schroeckh, T. Christopeit, G. Zandomeneghi, M. Fändrich, Protein Sci. 2005, 14, 1753.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmtVejtr4%3D&md5=b77ef6dce7fc2ee4cbde1f724b6e8bb1CAS |

[52]  B. Fuchs, A. Nimptsch, S. U. Rosmarie, J. Schiller, J. AOAC Int. 2008, 91, 1227.
         | 1:CAS:528:DC%2BD1cXht1ymtLjM&md5=bc5530262acec83aa369c6611ce19624CAS |

[53]  E. House, M. Mold, J. Collingwood, A. Baldwin, S. Goodwin, C. Exley, J. Alzheimer’s Dis. 2009, 18, 811.
         | 1:CAS:528:DC%2BD1MXhsFynsrfI&md5=bac3997902bec7fedec69c1a590fd2edCAS |

[54]  X. H. Yang, H. C. Huang, L. Chen, W. Xu, Z. F. Jiang, J. Alzheimer’s Dis. 2009, 18, 799.
         | 1:CAS:528:DC%2BD1MXhsFynsrnL&md5=45557043ee1e75ae6b7620dafc81e7c0CAS |

[55]  J. T. Pedersen, K. Teilum, N. H. H. Heegaard, J. Østergaard, H. W. Adolph, L. Hemmingsen, Angew. Chem. Int. Edit. 2011, 50, 2532.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXislagtrc%3D&md5=e065474a8df4dbbcba9f800eeaae29b0CAS |

[56]  M. Garvey, K. Tepper, C. Haupt, U. Knüpfer, K. Klement, J. Meinhardt, U. Horn, J. Balbach, M. Fändrich, Biochem. Biophys. Res. Commun. 2011, 409, 385.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnsV2js7k%3D&md5=1d8633ae34e9512604996b02adc51a73CAS |

[57]  M. T. Le, J. Gailer, E. J. Prenner, Biometals 2009, 22, 261.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjsFyktLk%3D&md5=39ac6f09abd8946ecff7730d322f8badCAS |