Journal of Biological Chemistry
Protein Structure and FoldingNegative Stain Single-particle EM of the Maltose Transporter in Nanodiscs Reveals Asymmetric Closure of MalK2 and Catalytic Roles of ATP, MalE, and Maltose*Conformational Dynamics of the Maltose Transporter
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This work was supported by Canadian Institutes of Health Research Grants 86693MOP (to I. R.) and 74525MOP (to F. D.), the Natural Sciences and Engineering Research Council of Canada Grant RGPIN-2014-04798 (to I. R.), a Bourse de Prestige from the Groupe d'Étude des Protéines Membranaires (which is supported by the Fonds de la Recherche en Santé du Québec) (to L. F.), and a summer fellowship from the Natural Sciences and Engineering Research Council of Canada Create Training Program in Bionanomachines (to J. I.). The authors declare that they have no conflicts of interest with the contents of this article.
This article contains supplemental Table S1 and Figs. S1–S7.
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The abbreviations used are:
- ABC
ATP-binding cassette
- NBD
nucleotide-binding domain
- TMD
transmembrane domain
- ATPγS
adenosine 5′-O-(thiotriphosphate)
- AMP-PNP
adenosine 5′-(β,γ-iminotriphosphate)
- PDB
Protein Data Bank
- BMOE
bismaleimidoethane.