Journal of Biological Chemistry
Editors' PicksA fluorescent timer reporter enables sorting of insulin secretory granules by age
Cited by (0)
This article contains supporting information.
Author contributions—B. Y., L. H., C. L., and D. R. L. data curation; B. Y., L. H., C. L., D. R. L., and M. A. K. formal analysis; B. Y., L. H., P. T., C. J. R., and M. A. K. methodology; B. Y. writing-original draft; B. Y. and M. A. K. project administration; L. H. and M. A. K. investigation; H. E. T., J. E. G., L. W., W. J. H., C. J. R., and M. A. K. resources; P. T. and M. A. K. supervision; P. T. and M. A. K. funding acquisition; M. A. K. conceptualization; M. A. K. writing-review and editing.
Funding and additional information—The work was supported by National Health and Medical Research Council (NHMRC) of Australia Grant GNT1139828. M. A. K. is supported by a Jennie Mackenzie Philanthropic Fellowship, University of Sydney.
Conflict of interest—CJR is a current employee of AstraZeneca and owns stock in the company.
Abbreviations—The abbreviations used are:
- GSIS
glucose-stimulated insulin secretion
- SG
secretory granule
- ISG
insulin SG
- HTRF
homogeneous time-resolved fluorescence
- FAOS
flow cytometer–assisted organelle sorting
- IBMX
3-isobutyl-1-methylxanthine
- DAPI
4′,6-diamidino-2-phenylindole
- TIRF
total internal reflection fluorescence
- ANOVA
analysis of variance.