Journal of Biological Chemistry
Computational BiologyGlobal analysis of adenylate-forming enzymes reveals β-lactone biosynthesis pathway in pathogenic Nocardia
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Author contributions—S. L. R., M. H. M., and L. P. W. conceptualization; S. L. R., S. J. P., T. P. S., and M. H. M. resources; S. L. R' B. R. T. data curation; S. L. R. and B. R. T. software; S. L. R. formal analysis; S. L. R., M. H. M., and L. P. W. funding acquisition; S. L. R., B. R. T., and S. J. P. validation; S. L. R., B. R. T., M. D. S., and S. J. P. investigation; S. L. R. visualization; S. L. R., B. R. T., and M. H. M. methodology; S. L. R. writing-original draft; S. L. R., T. P. S., M. H. M., and L. P. W. project administration; S. L. R., B. R. T., M. D. S., S. J. P., T. P. S., M. H. M., and L. P. W. writing-review and editing; M. H. M. and L. P. W. supervision.
Funding and additional information—S.L.R. is supported by the National Science Foundation Graduate Research Fellowship under NSF grant number 00039202 and a Graduate Research Opportunities Worldwide (GROW) fellowship to the Netherlands supported by the NSF and the Netherlands Organization for Scientific Research (NWO) grant number 040.15.054/6097 (to S. L. R. and M. H. M.).
Conflict of interest—M. H. M. is a co-founder of Design Pharmaceuticals and on the scientific advisory board of Hexagon Bio.
Present address for Serina L. Robinson: Institute of Microbiology, ETH Zürich, Zürich, Switzerland.
Abbreviations—The abbreviations used are:
- NRPS
nonribosomal peptide synthetase
- A domain
adenylation domain
- SACS
short-chain acyl-CoA synthetase
- MACS
medium-chain acyl-CoA synthetase
- LACS
long-chain acyl-CoA synthetase
- FAAL
fatty acyl-AMP ligase
- LUC
luciferase
- BLS
β-lactone synthetase
- ARYL
aryl-CoA ligase
- pHMM
profile Hidden Markov Model
- FSI
fatty acyl-AMP ligase-specific insertion
- AUROC
area under the receiver operating characteristic curve
- aa
amino acid(s).