Journal of Biological Chemistry
Signal TransductionCaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins
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Author contributions—C. G. L., M. T. O., N. X. Y. L., T. A. D., J. S. O., B. E. K., and J. W. S. conceptualization; C. G. L., T. A. D., S. G., K. L., M. W. P., J. S. O., B. E. K., and J. W. S. supervision; C. G. L., B. E. K., and J. W. S. funding acquisition; C. G. L., M. T. O., K. R. N., L. M. M., U. D., A. H., N. X. Y. L., T. A. D., S. G., K. L., M. W. P., J. S. O., B. E. K., and J. W. S. investigation; C. G. L., M. T. O., K. R. N., L. M. M., U. D., A. H., N. X. Y. L., T. A. D., S. G., K. L., M. W. P., J. S. O., and B. E. K. methodology; C. G. L., M. T. O., B. E. K., and J. W. S. writing-original draft; C. G. L., B. E. K., and J. W. S. project administration; C. G. L., M. T. O., L. M. M., A. H., N. X. Y. L., T. A. D., S. G., K. L., M. W. P., J. S. O., B. E. K., and J. W. S. writing-review and editing; M. T. O. formal analysis.
Funding and additional information—This work was supported by National Health and Medical Research Council Grants 1138102 (to J. W. S.) and 1145265 (to J. S. O.) and the Jack Brockhoff Foundation Grant JBF-4206, 2016 (to C. G. L.). C. G. L. is a National Health and Medical Research Council Early Career Research Fellow and M. W. P. a National Health and Medical Research Council Senior Principal Research Fellow. B. E. K. was a National Health and Medical Research Council Fellow supported by the Australian Research Council (DP170101196). This project was supported in part by the Victorian Government's Operational Infrastructure Support Program. MS data were acquired at the Mass Spectrometry and Proteomics Facility, Bio21, University of Melbourne. We acknowledge the funding support of the Australian Cancer Research Foundation toward the ACRF Rational Drug Discovery Centre.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Present address for Toby A. Dite: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Abbreviations—The abbreviations used are:
- CaM
calmodulin
- CaMK
Ca2+-calmodulin dependent protein kinase
- PKA
protein kinase A
- AMPK
AMP-activated protein kinase
- IBMX
3-isobutyl-1-methylxanthine
- GLP
glucagon-like peptide
- ANOVA
analysis of variance
- SPR
surface plasmon resonance.
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These authors contributed equally to this work.