Journal of Biological Chemistry
Cell BiologyKnockout mouse models reveal the contributions of G protein subunits to complement C5a receptor–mediated chemotaxis
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Author contributions—E. v. d. B., S. W., and P. J. H. formal analysis; E. v. d. B., B. A., M. H., and P. J. H. investigation; M. H. visualization; A. C. B. and P. J. H. writing-original draft; N. W., G. I., and T. M. W. resources; P. J. H. conceptualization; P. J. H. data curation; P. J. H. supervision; P. J. H. funding acquisition; P. J. H. methodology; P. J. H. project administration.
Funding and additional information—This work was supported by Deutsche Forschungsgemeinschaft (DFG) Grant HA 3271/3-2 (to P. J. H.). T. M. W. is supported by NCI, National Institutes of Health, Grant CA192381. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- GPCR
G protein–coupled receptor
- C5aR
complement C5a receptor
- PTX
pertussis toxin
- AM
acetoxymethyl
- dKO
double knockout
- ER
endoplasmic reticulum
- PLC-β
phospholipase C-β
- IP3
inositol 1,4,5-trisphosphate
- PIP2
phosphatidylinositol 4,5-bisphosphate
- PI3K
phosphoinositide 3-kinase
- PIP3
phosphatidylinositol 3,4,5-trisphosphate
- 2D
two-dimensional
- FCS
fetal calf serum
- MPG
N-(2-mercaptopropionyl)glycine
- P2Y2R
P2Y2 receptor
- GEF
guanine nucleotide exchange factor
- GAP
GTPase–activating protein.