Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-27T09:49:29.868Z Has data issue: false hasContentIssue false
  • English
  • Français

Factors affecting the absorption of iron from Fe(III)EDTA

Published online by Cambridge University Press:  09 March 2007

A. P. MacPhail ,
T. H. Bothwell ,
J. D. Torrance ,
D. P. Derman ,
W. R. Bezwoda ,
R. W. Charlton  and
Fatima Mayet
A. P. MacPhail
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
T. H. Bothwell
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
J. D. Torrance
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
D. P. Derman
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
W. R. Bezwoda
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
R. W. Charlton
Affiliation:
Joint University/South African MRC Iron and Red Cell Metabolism Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
Fatima Mayet
Affiliation:
Department of Medicine, University of Natal, Durban, South Africa
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The modification of iron absorption from Fe(III)EDTA by agents known to promote or inhibit absorption was examined in 101 volunteer multiparous Indian women. Fe absorption from Fe(III)EDTA was compared with absorption of intrinsic food Fe in a further twenty-eight subjects. Finally the urinary excretion of radio-Fe after oral administration of 59Fe(III)EDTA was studied in twenty-four subjects and evidence of intraluminal exchange of Fe was examined.

2. Fe absorption from maize porridge fortified with Fe(III)EDTA was more than twice that from porridge fortified with FeSO4. 7H2O.

3. Although bran decreased Fe absorption from FeSO4. 7H2O approximately 11-fold, it had no significant effect on Fe absorption from Fe(III)EDTA. Nevertheless tea, which is a more potent inhibitor of Fe absorption, decreased absorption from Fe(III)EDTA 7-fold.

4. Fe absorption from Fe(III)EDTA given in water was only increased 40% by addition of 3 mol ascorbic acid/mol Fe but by 7-fold when the relative proportions were increased to 6:1. This enhancing effect was blunted when the Fe(III)EDTA was given with maize porridge. In these circumstances, an ascorbate:iron value of 3:1 (which doubles absorption from FeSO4. 7H2O) produced no significant increase in Fe absorption, while a value of 6:1 produced only a 2.5-fold increase.

5. Fe absorption from Fe(III)EDTA was not altered by addition of maize porridge unless ascorbic acid was present.

6. Less than 1% of 59Fe administered as 59Fe(III)EDTA was excreted in the urine and there was an inverse relationship between Fe absorption and the amounts excreted (r 0.58, P > 0.05).

7. Isotope exchange between 55Fe(III)EDTA and 59FeSO4. 7H2O was demonstrated by finding a similar relative value for the two isotopes in urine and erythrocytes when the two labelled compounds were given together orally. This finding was confirmed by in vitro studies, which showed enhanced 59Fe solubilization from 59FeSO4. 7H2O in maize porridge when unlabelled Fe(III)EDTA was added.

8. Although Fe absorption from Fe(III)EDTA was marginally higher it appeared to form a common pool with intrinsic food iron in most studies. It is postulated that the mechanism whereby Fe(III)EDTA forms a common pool with intrinsic food Fe differs from that occurring with simple Fe salts. When Fe is present in the chelated form it remains in solution and is relatively well absorbed because it is protected from inhibitory ligands. Simple Fe salts, however, are not similarly protected and are absorbed as poorly as the intrinsic food Fe.

9. It is concluded that Fe(III)EDTA may be a useful compound for food fortification of cereals because the Fe is well absorbed and utilized for haemoglobin synthesis. The substances in cereals which inhibit absorption of simple Fe salts do not appear to inhibit absorption of Fe from Fe(III)EDTA.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 45 , Issue 2 , March 1981 , pp. 215 - 227
Copyright
Copyright © The Nutrition Society 1981

References

REFERENCES

Bezwoda, W., Charlton, R., Bothwell, T., Torrance, J. & Mayet, F. (1978). J. Lab. clin. Med. 92, 108.Google Scholar
Björn-Rasmussen, E. & Hallberg, L. (1972). Am. J. clin. Nutr. 25, 317.CrossRefGoogle Scholar
Björn-Rasmussen, E. & Hallberg, L. (1974). Nutr. Metab. 16, 94.CrossRefGoogle Scholar
Bothwell, T. H., Charlton, R. W., Cook, J. D. & Finch, C. A. (1979). In Iron Metabolism in Man, p. 443. Oxford: Blackwell.Google Scholar
Cook, J. D., Layrisse, M., Martinez-Torres, C., Walker, R. B., Monsen, E. & Finch, C. A. (1972). J. clin. Invest. 51, 805.CrossRefGoogle Scholar
Deppe, W. M., Joubert, S. M. & Naidoo, P. (1978). J. clin. Path. 31, 145.CrossRefGoogle Scholar
Derman, D., Sayers, M., Lynch, S. R., Charlton, R. W. & Bothwell, T. H. (1977). Br. J. Nutr. 38, 261.CrossRefGoogle Scholar
Disler, P. B., Lynch, S. R., Charlton, R. W., Bothwell, T. H., Walker, R. B. & Mayet, F. (1975). Br. J. Nutr. 34, 141.CrossRefGoogle Scholar
Disler, P. B., Lynch, S. R., Charlton, R. W., Torrance, J. D., Bothwell, T. H., Walker, R. B. & Mayet, F. (1975). Gut 16, 193.CrossRefGoogle Scholar
Hallberg, L., Björn-Rasmussen, E., Garby, L., Pleehachinda, R. & Suwanik, R. (1978). Am. J. clin. Nutr. 31, 1403.CrossRefGoogle Scholar
Herbert, V., Gottlieb, C. W. & Lau, K. S. (1967). J. nucl. Med. 8, 529.Google Scholar
Hussain, R., Walker, R. B., Laynsse, M., Clark, P. & Finch, C. A. (1965). Am. J. clin. Nutr. 16, 464.CrossRefGoogle Scholar
International Commission for Radiation Protection (1960). Report of Committee 11 on Permissible Dose of Internal Radiation 1959. ICRP Publication no. 2 Oxford: Pergamon Press.Google Scholar
International Committee for Standardisation in Haematology (1978). Br. J. Huemat. 38, 291.CrossRefGoogle Scholar
International Nutritional Anemia Consultative Group (1977). Guidelines for the Eradication of Iron Deficiency Anaemia, p. 16. New York: Nutrition Foundation.Google Scholar
Jacobs, A., Miller, F., Wonvood, M., Beamish, M. R. & Wardrop, C. A. (1972). Br. med. J. iv, 206.CrossRefGoogle Scholar
Katz, J. H., Zoukis, M., Hart, W. L. & Dern, R. J. (1964). J. Lab. clin. Med. 63, 885.Google Scholar
Kuhn, I. N., Monsen, E. R., Cook, J. D. & Finch, C. A. (1968). J. Lab. clin. Med. 71, 715.Google Scholar
Layrisse, M., Cook, J. D., Martinez-Torres, C., Roche, M., Kuhn, I. N., Walker, R. B. & Finch, C. A. (1969). Blood 33, 430.CrossRefGoogle Scholar
Layrisse, M. & Martinez-Torres, C. (1971). Prog. Haemat. 7, 137.Google Scholar
Layrisse, M. & Martinez-Torres, C. (1977). Am. J. clin. Nutr. 30, 1166.CrossRefGoogle Scholar
Layrisse, M., Martinez-Torres, C., Cook, J. D., Walker, R. & Finch, C. A. (1973). Blood 41, 333.CrossRefGoogle Scholar
Lorber, L. (1927). Biochem. Z. 181, 391.Google Scholar
Martinez-Torres, C., Romano, E. L., Renzi, M. & Layrisse, M. (1979). Am. J. clin. Nutr. 32, 809.CrossRefGoogle Scholar
Mayet, F. G. H., Adams, E. B., Moodley, T., Kleber, E. E. & Cooper, S. K. (1972). S. Ajr. med. J. 46, 1427.Google Scholar
Sawyer, D. T. & McKinnie, J. M. (1960). J. Am. Chem. Soc. 82, 4191.CrossRefGoogle Scholar
Sayers, M. H., Lynch, S. R., Charlton, R. W. & Bothwell, T. H. (1974). Br. J. Nutr. 31, 367.CrossRefGoogle Scholar
Sayers, M. H., Lynch, S. R., Charlton, R. W., Bothwell, T. H., Walker, R. B. & Mayet, F. (1974). Br. J. Huemat. 28, 483.CrossRefGoogle Scholar
Sayers, M. H., Lynch, S. R., Jacobs, P., Charlton, R. W., Bothwell, T. H., Walker, R. B. & Mayet, F. (1973). Br. J. Haemat. 24, 209.CrossRefGoogle Scholar
Will, J. J. & Vilter, R. W. (1954). J. Lab. clin. Med. 44, 499.Google Scholar