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Ontogeny of brush border carbohydrate digestion and uptake in the chick

Published online by Cambridge University Press:  09 March 2007

David Sklan ,
Asaf Geyra ,
Elad Tako ,
Orit Gal-Gerber  and
Zehava Uni
David Sklan*
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Asaf Geyra
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Elad Tako
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Orit Gal-Gerber
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Zehava Uni
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
*
*Corresponding author: Professor D. Sklan, fax +972 8 9489865, email sklan@agri.huji.ac.il
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Abstract

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Ingestion of carbohydrates from the small intestine is the major route of energy supply in animals. In mammals these functions develop both pre- and postnatally and are coordinated for the sucking period. In birds, the physiological requirements are different and hatchlings ingest diets rich in complex carbohydrates soon after hatching. The present study examined the ontogeny of intestinal carbohydrate uptake in the chicken. The expression of mRNA for a brush border enzyme, sucrase–isomaltase (SI), which is critical in disaccharide digestion, was determined, together with that of the Na–glucose transporter (SGLT)-1, which is the major apical glucose transporter, In addition, the homeobox gene cdx, which is involved in inducing SI expression in mammals was examined. It was found that the expression of cdxA mRNA and cdxA protein increased from day 15 of incubation until hatch, after which further changes were small. CdxA protein was shown to bind to the promoter region of SI in the chick indicating that cdxA is similar to the mammalian cdx2. The mRNA of SI was observed at 15 d incubation, increased from 17 d of incubation to a peak on day 19, decreased at hatch and had a further peak of expression 2 d post-hatch. In contrast, the mRNA of SGLT-1 was not detected until 19 d of incubation when a major peak of expression was observed followed by a decrease to low levels at hatch and small increases post-hatch. It appears that both SI and SGLT-1 mRNA are expressed before hatch in the chick, but the ontogeny of expression is controlled by different mechanisms.

Keywords

Small intestineOntogenyCarbohydrateChick
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 6 , June 2003 , pp. 747 - 753
Copyright
Copyright © The Nutrition Society 2003

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