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Evaluation of genetic stability in cryopreserved Prunus

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Summary

The evaluation of the genetic stability of Prunus Ferlenain plants regenerated from cryopreserved apices was investigated. The analysis of plants recovered from frozen material was performed at the phenotypic (developmental competence), cytological (chromosomal preparations) and molecular level [random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP)]. No genetic change was detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material, including leaves of the mother tree kept in an orchard and vitroplantlets regenerated from non-frozen apices. This result suggested that the procedure used for Prunus cryopreservation could be used for long-term conservation. The relevance of each strategy for the genetic stability evaluation of the cryopreserved material is discussed.

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire Végétale, Université de Bordeaux 1, Av. des Facultés, 33405, Talence, France

    B. Helliot & M. T. De Boucaud

  2. Unité de Recherches sur les Espèces Fruitières et la Vigne, INRA, BP81, 33883, Villenave d'Ornon Cedex, France

    D. Madur & E. Dirlewanger

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  1. B. Helliot
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  2. D. Madur
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  3. E. Dirlewanger
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  4. M. T. De Boucaud
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Correspondence to E. Dirlewanger.

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Helliot, B., Madur, D., Dirlewanger, E. et al. Evaluation of genetic stability in cryopreserved Prunus . In Vitro Cell Dev Biol -Plant 38, 493–500 (2002). https://doi.org/10.1079/IVP2002325

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  • DOI: https://doi.org/10.1079/IVP2002325

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