Summary
An efficient and rapid micropropagation system was developed for a food and medicinally important endangered shrub, Decalepis hamiltonii (‘swallow root’), through shoot multiplication. The influence of 2.5–7.5 μM isopentenyladenine (2iP), 4.4–17.7 μM 6-benzyladenine, 2.3–4.7 μM kinetin, 2.8–6.8 μM thidiazuron, and 2.3–11.4 μM zeatin alone and in combination with 0.3–0.9 μM indole-3-acetic acid (IAA) on in vitro multiple shoot production was studied. The maximum number of multiple shoots (6.5±0.4) was induced from shoot tips cultured on agar-based Murashige and Skoog (MS) medium containing 4.9 μM 2iP. But, both zeatin (9.1 μM) and kinetin (4.7 μM) in combination with IAA (0.6 μM) were able to produce a maximum of 5.0±0.4 and 5.1±0.4 multiple shoots, respectively. Further elongation of shoots and adventitious shoot formation was obtained on medium containing 2.5 μM 2iP and 0.3 μM gibberellic acid. Elongated shoots were separated and rooted on MS medium supplemented with 9.8μM indole-3-butyric acid (IBA) and various phenolic compounds within 5–6 wk. Phloroglucinol and salicylic acid interaction with IBA stimulated in vitro rooting of shoots. Successful field transfer was achieved in rooted plantlets.
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Bais, H. P.; George, J.; Ravishankar, G. A. In vitro propagation of Decalepis hamiltonii Wight & Arn., an endangered shrub, through axillary bud cultures. Curr. Sci. 79:408–410; 2000.
George, J.; Bais, H. P.; Ravishankar, G. A. Optimization of media constituents for shoot regeneration from leaf callus cultures of Decalepis hamiltonii Wight & Arn. HortScience 35:296–299; 2000.
George, J.; Pereira, J.; Divakar, S.; Udaysankar, K.; Ravishankar, G. A. A. method for preparation of active fraction from the root of Decalepis hamiltonii, useful as bioinsecticides. Indian patent no. 1301/Del/98; 1998.
George, J.; Udaysankar, K.; Keshava, N.; Ravishankar, G. A. Antibacterial activity of supercritical extract from Decalepis hamiltonii roots. Fitoterapia 70:172–174; 1999.
Hess, C. E. Characterization of the rooting cofactors extracted from Hedera helix L. and Hibiscus rosa-sinensis L. Proc. 16th Int. Hort. Congr. 1962:382–388.
Kling, G. J.; Meyer, M. M. Effects of phenolic compounds and IAA on adventitious roots formation in cuttings of Phaseolus aureus, Acer saccharinum and Acer griseum. HortScience 18:352–354; 1983.
Murashige, T.; Skoog, F. A revised medium for raid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Phadke, N. Y.; Gholap, A. S.; Subbalakshmi, G.; Ramakrishnan, K. Essential oil of Decalepis hamiltonii as an antimicrobial agent. J. Food Sci. Technol. 31:472–475; 1994.
Reed, B. M.; Abdelnour-Esquivel, A. The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. HortScience 26:1320–1322; 1991.
Sarkar, D.; Naik, P. S. Phloroglucinol enhances growth and rate of axillary shoot proliferation in potato shoot tip culture in vitro. Plant Cell Tiss. Organ Cult. 60:139–149; 2000.
Still, S. M.; Dirr, M. A.; Gartener, J. B. Phytotoxic effects of several bark extracts on mung bean and cucumber growth. J. Am. Soc. Hort. Sci. 101:34–37; 1976.
Wawrosch, C.; Malla, P. R.; Kopp, B. Clonal propagation of Lilium nepalense D Don, a threatened medicinal plant of Nepal. Plant Cell Rep. 20:285–288; 2001.
Wealth of India. Raw materials: Decalepis hamiltonii Wight & Arn., vol. 3. New Delhi: CSIR; 1990:161–162.
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Giridhar, P., Gururaj, H.B. & Ravishankar, G.A. In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight & Arn., a threatened plant endemic to Southern India. In Vitro Cell.Dev.Biol.-Plant 41, 77–80 (2005). https://doi.org/10.1079/IVP2004600
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DOI: https://doi.org/10.1079/IVP2004600