Summary
Guar gum, a galactomannan derived from the endosperms of Cyamposis tetragonoloba, has been successfully used as a sole gelling agent for plant tissue culture media. Its suitability as a gelling agent was demonstrated by using guar gumgelled media for in vitro seed germination of Linum usitatissimum and Brassica juncea, in vitro axillary shoot proliferation in nodal explants of Crataeva nurvala, rooting of regenerated shoots of the same, in vitro androgenesis in anther cultures of Nicotiana tabacum, and somatic embryogenesis in callus cultures of Calliandra tweedii. The media used for these were gelled with either guar gum (2, 3, or 4%) or agar (0.9%). Guar gum-gelled media, like agar media, supported all these morphogenic responses. Rather, axillary shoot proliferation, rhizogenic and embryogenic responses were better on guar gum-gelled media than on agar media.
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References
Anonymous, Gums, food resources, http://food.orst.edu/; 2001.
Babbar, S. B.; Jain, N. Isubgol as an alternative gelling agent for plant tissue culture media. Plant Cell Rep. 17:318–322; 1998.
Chu, C. C. The N6 medium and its applications to anther culture of cereal crops In: Proc. Symp. Plant Tiss. Cult., Beijing: Science Press; 1978:43–59.
Creager, J. G.; Black, J. G.; Davison, V. E. Microbiology: principles and applications. Englewood Cliffs, NJ: Prentice Hall; 1990.
Eyre, M. J.; Caswell, S. C. Sterile culture of Rotylenchulus reniformis on tomato root with gellan gum as a supporting medium. J. Nematol. 23:229–231; 1991.
Henderson, W. E.; Kinnersley, A. M. Corn starch as an alternative gelling agent for plant tissue culture media. Plant Cell Tiss. Organ Cult. 15:17–22; 1988.
Jain, N.; Babbar, S. B. Gum katira—a cheap gelling agent for plant tissue culture media. Plant Cell Tiss. Organ Cult. 71:223–229; 2002.
Jain, R.; Babbar, S. B. Abstract. Proc. 4th Int. Plant Tiss. Cult. Conf. Dhaka; 2001:97.
Knop, W. Quantitative Untersuchung Über die Ernährungsprozesse der Pflanzen. Landwirtsch Vers. Stn. 30:292–294; 1865.
Kumar, S.; Agrawal, V.; Gupta, S. C. Somatic embryogenesis in woody legume Calliandra tweedii. Plant Cell Tiss. Organ Cult. 71:77–80; 2002.
McLachlan, J. Macroalgae (seaweeds): industrial resources and their utilization. Plant Soil. 89:137–157; 1985.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Nitsch, J. P. Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404; 1969.
Pasqualetto, P. L.; Zimmerman, R. H.; Fordham, I. M. The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tiss. Organ Cult. 14:31–40; 1988.
Pelezar, M. J. Jr.; Chan, E. C. S.; Krieg, N. R. Microbiology. Singapore: McGraw-Hill Book Co.; 1986.
Redenbaugh, K.; Fujii, J. A. A.; Slade, D. Hydrated coatings for synthetic seeds. In: Redenbaugh, K., ed. Synseeds: applications of synthetic seeds to crop improvement. Boca Raton, FL: CRC Press; 1993:35–46.
Sunderland, N.; Dunwell, J. M. Anther and pollen culture. In: Street, H. E., ed. Plant tissue and cell culture. Oxford: Blackwell Scientific Publishers; 1977:223–264.
Walia, N.; Sinha, S.; Babbar, S. B. Micropropagation of Crataeva nurvala. Biol. Plant. 46:181–185; 2003.
White, P. R. Controlled differentiation in plant tissue culture. Bull. Torrey Bot. Club 66:507–513; 1939.
Windholz, M.; Budavari, S.; Blumetti, R. F.; Otterbein, E. S. The Merck Index: an encyclopedia of chemicals, drugs and biologicals, 3rd edn. Rahway, NJ: Merck and Co. Inc.: 1983.
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Babbar, S.B., Jain, R. & Walia, N. Guar gum as a gelling agent for plant tissue culture media. In Vitro Cell.Dev.Biol.-Plant 41, 258–261 (2005). https://doi.org/10.1079/IVP2005628
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DOI: https://doi.org/10.1079/IVP2005628