Summary
We report a protocol for somatic embryogenesis and plantlet regeneration of Buchanania lanzan Spreng (Family—Anacardiaceae), which is a tropical fruit tree widely distributed in the dry forests of India. Calluses were initiated from immature zygotic embryos cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzyladenine (BA) and/or 1-naphthaleneacetic acid (NAA). The highest frequency (60%) of somatic embryo induction was obtained in cultures grown on MS medium fortified with 4.53 μM 2,4-D, 5.32 μM NAA and 4.48 μM BA. The medium supplemented with 15 μM abscisic acid (ABA) was most effective for maturation and germination of somatic embryos. This is the first report on somatic embryogenesis in B. lanzan, which may be helpful for in vitro propagation, ex situ conservation and genetic manipulation of this species.
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References
Ammirato, P. V. Embryogenesis. In: Evans, D. V.; Sharp, W. R.; Ammirto, P. V.; Yamada, Y., eds. Handbook of plant cell culture, vol. 1. Techniques for propagation and breeding. New York: Macmillan Publishing Co.; 1983:82–123.
Dong, J. Z.; Bock, C. A.; Dunstan, D. I. Influences of altered phytohormone use on endogenous ABA and mRNA populations during white spruce (Picea glauca) somatic embryo culture. Tree Physiol. 17:53–57; 1997.
Eapen, S.; George, L. Somatic embryogenesis in peanut: influence of growth regulators and sugars. Plant Cell Tiss. Organ Cult 35:151–156; 1993.
Emons, A. M. C.; Droppers, A. S.; Toorn, C. V. D. The influence of sucrose, mannitol, l-proline, abscisic acid and gibberellic acid on the maturation of somatic embryos of Zea mays L. from suspension cultures. J. Plant Physiol. 142:597–604; 1993.
Fulzele, D. P.; Satdeve, R. K. Somatic embryogenesis, plant regeneration, and the evaluation of camptothecin content in Nothapodytes foetida. In Vitro Cell. Dev. Biol.—Plant. 39:212–216; 2003.
Kaushik, P.; Dhiman, A. K. Buchanania lanzan In: Medicinal plants and raw drugs of India. Dehra-Dun: Bishen Singh Mahendra Pal Singh; 1999:175–177.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Parkash, R.; Chaudhari, D. C.; Negi, S. S. Buchanania lanzan In: Propagation practice of important Indian trees. Dehra-Dum; International Book Distributors; 1991: 144–146.
Singh, J.; Banerjee, S. K.; Francis, A. Vegetative propagation of Bucchanania lanzen Spreng G. root cutting. Ind. For. 6:700–704; 2002.
Sumathi, R.; Malliga, P.; Yashodha, R.; Gurumurthy, K. Enhanced somatic embryogenesis and plant regeneration in bamboo (Dendrocalamus strictus). Plant Cell Biotechnol. Mol. Biol. 4:9–16; 2003.
Torres, A. C.; Ze, N. M.; Cantliffe, D. J. Abscissic acid and osmotic induction of synchronous somatic embryo development of sweet potato. In Vitro Cell. Dev. Biol.—Plant 37:262–267; 2001.
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Sharma, P., Koche, V., Quraishi, A. et al. Somatic embryogenesis in Buchanania lanzan spreng. In Vitro Cell.Dev.Biol.-Plant 41, 645–647 (2005). https://doi.org/10.1079/IVP2005680
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DOI: https://doi.org/10.1079/IVP2005680