Summary
The purpose of this study was to develop an efficient micropropagation system for Mucuna pruriens, an important medicinal plant in India. A range of cytokinins was investigated for multiple shoot regeneration with cotyledonary node explants from 7-d-old aseptic seedlings. Of all the cytokinins, 6-benzyladenine (BA), kinetin (KIN) and 2-isopentenyl adenine (2-iP) tested in Murashige and Skoog medium (MS), BA was the most effective and 5.0 μM was found to be optimum for inducing maximum shoots. Medium types, medium strength and pH were also investigated for induction and proliferation of shoots. The highest efficiency of shoot proliferation was observed in 5.0 μM BA and 0.5 μM α-naphthalene acetic acid (NAA) in half-strength MS medium at pH 5.8. The best condition for rooting was half-strength MS medium solidified with agar and with 2.0 μM indole-3-butyric acid (IBA). After rooting, the plantlets were transferred to plastic pots filled with sterile soilrite where 90% grew and all exhibited normal development.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amo-Marco, J. B.; Ibanez, M. R. Micropropagation of Lilium cavanillesii Erben, a threatened statice, from inflorescence stems. Plant Growth Regul. 24:49–54; 1998.
Anis, M.; Faisal, M. In vitro regeneration and mass propagation of Psoralea corylifolia—an endangered medicinal plant. Indian J. Biotechnol. 4:261–264; 2005.
Anonymous. The wealth of India: a dictionary of Indian raw materials and industrial products, vol. 4. New Delhi: Publications and Information Directorate, CSIR; 2003:166–167.
Arora, R.; Bhojwani, S. S. In vitro propagation and low temperature storage of Saussurea lappa C.B. Clarke—an endangered medicinal plant. Plant Cell Rep. 8:44–47; 1989.
Bell, E. A.; Janzen, D. H. Medicinal and ecological consideration of l-Dopa and 5-HTP in seeds. Nature 229:136–137; 1971.
Daxenbichler, M. E.; Etten, C. H.; Van Hallman, E. A.; Earle, F. R.; Barclay, A. S. Seeds as sources of l-Dopa. Med. Chem. 14:463–465; 1971.
Faisal, M.; Anis, M. Rapid in vitro propagation of Rauvolfia tetraphylla L.—an endangered medicinal plant. Physiol. Mol. Biol. Plants 8:295–299; 2002.
Faisal, M.; Anis, M. Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell Tiss. Organ Cult. 75:125–129; 2003.
Faisal, M.; Singh, S.; Anis, M. In vitro regeneration and plant establishment of Tylophora indica (Burm. f.) Merill: petiole callus culture. In Vitro Cell. Dev. Biol. Plant 41:511–515; 2005.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrients requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.
Hutchinson, J. F. In vitro propagation of Dionaea muscipula Ellis (venus fly trap). Sci. Hortic. 22:189–194; 1984.
Joshi, M.; Dhar, U. In vitro propagation of Saussurea aobvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep. 21:933–939; 2003.
Jusaitis, M. Micropropagation of adult Swaisona formosa Leguminosae: Papilionoideae: Galegeae. In Vitro Cell. Dev. Biol. Plant 33:213–220; 1997.
Kaneda, Y.; Tabei, S.; Nishimura, S.; Harada, K.; Akihama, T.; Kitamura, K. Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybean [Glycine max (L.) Merr]. Plant Cell Rep. 17:8–12; 1997.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.
Parliman, B. J.; Evans, P. T.; Rupert, E. A. Tissue culture of single rhizome explant of Dionaea muscipula Ellis ex. L., the venus flytrap for rapid asexual propagation. HortScience 107:305–310; 1982.
Pattnaik, S. K.; Chand, P. K. In vitro propagation of the medicinal herbs Ocimum americanum L. syn., O. canum Sims (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Rep. 15:846–850; 1996.
Phillips, G. C.; Collins, G. B. In vitro tissue culture of selected legumes and plant regeneration from callus of red clover. Crop Sci. 19:59–64; 1979.
Rao, J. V. S.; Vishnupriya, K. S. In vitro response of embryo axis of Entada phaseoloides Merrill. Phytomorphology 52:97–102; 2002.
Reddy, P. S.; Gopal, R. G.; Sita, L. G. In vitro multiplication of Gymnema sylvestre R. Br. An important medicinal plant. Current Sci. 75:843–845; 1998.
Saxena, C.; Palai, S. K.; Samataray, S.; Rout, G. R.; Das, P. Plant regeneration from callus cultures of Psoralea corylifolia Linn. Plant Growth Regul. 22:13–17; 1997.
Singh, U.; Wadhwani, A. N.; Johri, B. M. Dictionary of economic plants in India. New Delhi: Indian Council of Agricultural Research; 1996:45–146.
Stefaan, P.; Werbrouck, O.; Debergh, P. C. Applied aspects of plant regeneration. In: Dixon, R. A.; Gonzales, R. A., eds. Plant cell culture: a practical approach. Oxford: Oxford University Press; 1994:127–145.
Sudha, G. C.; Seeni, S. In vitro multiplication and field establishment of Adhatoda beddomii CB Clarke, a rare medicinal plant. Plant Cell Rep. 10:67–70; 1994.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Faisal, M., Siddique, I. & Anis, M. An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants. In Vitro Cell.Dev.Biol.-Plant 42, 59–64 (2006). https://doi.org/10.1079/IVP2005717
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2005717