The following article is Open access

STED microscopy resolves nanoparticle assemblies

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Published 22 June 2006 Published under licence by IOP Publishing Ltd
, , Citation K I Willig et al 2006 New J. Phys. 8 106 DOI 10.1088/1367-2630/8/6/106

1367-2630/8/6/106

Abstract

We demonstrate the ability of stimulated emission depletion (STED) microscopy, a far-field fluorescence imaging technique with diffraction-unlimited resolution, to reveal the spatial order of fluorescent nanoparticles. Unlike its confocal counterpart, here STED microscopy resolves the arrangements of densely packed 40 nm beads, supramolecular aggregates in a cell membrane, and colloidal nanoparticles. Both raw and linearly deconvolved data disclose unprecedented details of both biological and non-biological nanopatterns.

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