Molecular analysis of candida albicans Secreted Aspartyl Proteinase 3 (SAP3) Indonesian isolate

Secreted aspartyl proteinase 3 (SAP3) is one of the important virulence factor of Candida albicans, an opportunistic fungal pathogen responsible for superficial and life-threatening infections in humans. SAP3 also contribute to the pathogenicity of Candida albicans; therefore, become a target for therapy and vaccine development. However, data of the Candida albicans SAP3 protein from Indonesian isolate is limited. The present study aimed to molecular analysis the Candida albicans SAP3 cloned from Indonesian isolate. To study Candida albicans SAP3 protein from Indonesian isolate, a Candida albicans genomic DNA was isolated from a Javanese HIV/AIDS patient. A complete coding sequence of Candida albicans SAP3 gene was cloned and sequenced. The sequencing results were subjected to bioinformatics analysis. Physicochemical analysis revealed the molecular weight (Mw), estimated half-life, instability index, isoelectric point, aliphatic index, and hydrophilicity of the SAP3 protein. The antigenicity and epitope prediction also had been discussed. The results of this study would contribute information about Candida albicans SAP3 and benefits for further works willing to develop therapeutic and vaccine against the fungal.