Application of Models to Determination of Nutrient Requirements: Experimental Techniques Employing Tracers1

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Abstract

This paper concerns some issues involved in the design of tracer experiments for the development of whole-body compartmental models of nutrient metabolism. It focuses on tracer administration protocol, use of multiple tracers, sampling strategy, measurement types and experiment duration in a pragmatic approach to obtaining data suitable for analysis and interpretation with use of models.

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2To whom correspondence should be addressed: University of Pennsylvania, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348

1

Presented as part of a symposium: Application of Models to Determination of Nutrient Requirements, given at the 75th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlanta, GA, April 23,1991. The symposium was sponsored by the American Institute of Nutrition. Guest editor for this symposium was S. P. Coburn, Biochemistry Department, Fort Wayne Developmental Center, Fort Wayne, IN

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