1887

Abstract

The pMGA multigene family encodes variant copies of the cell surface haemagglutinin of . Quantitative Southern blotting, using an oligonucleotide probe complementary to a region conserved in the leader sequence of all known pMGA genes, was used to estimate the number of members of the family in the genome of seven strains of . The number of copies estimated to be present in the genome varied from 32 in strain F to 70 in strain R, indicating that the pMGA gene family may be second in size only to the tRNA family among prokaryotes. If all members of the pMGA family are of similar length to those which have been characterized, a minimum of 79 kb (7.7%) of the genome of strain S6, 82 kb (8.2%) of PG31 and 168 kb (16%) of the genome of strain R is dedicated to encoding variants of the same haemagglutinin. The GAA repeat motif identified in the intergenic region between all characterized pMGA genes appeared to be a feature common to most, if not all, pMGA genes, and furthermore probably exclusive to them. The genomic locations of members of the pMGA family were determined by PFGE and Southern blot hybridization of strain S6. The hybridizing regions were localized to four separate regions on the chromosome. The pMGA genes are likely to be predominantly arranged as tandem repeats within these regions, similar to the restricted regions for which the genomic sequence has been determined.

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1996-06-01
2024-03-28
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References

  1. Bencina D., Kleven S.H., Elfaki M.G., f Snoj A., Dove P., Dorrer D., Russ I. Variable expression of epitopes on the surface of Mycoplasma gallisepticum demonstrated with monoclonal antibodies. Avian Pathol 1994; 23:19–36
    [Google Scholar]
  2. Chen X. Physical maps of genomes of Mycoplasma gallisepticum PG31 and S6 with some functional loci 1992 PhD thesis, University of Melbourne;
    [Google Scholar]
  3. Church G.M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci USA 1984; 81:1991–1995
    [Google Scholar]
  4. Deckers H.M., Voordouw G. Identification of a large family of genes for putative chemoreceptor proteins in an ordered library of the Desulfovihrio vulgaris Hildenborough genome. J Bacteriol 1994; 176:351–358
    [Google Scholar]
  5. Del Sal G., Manfidetti G., Schneider C. A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res 1988; 16:9878
    [Google Scholar]
  6. Edward D.E.F.F., Kanarek A.D. Organisms of the pleuropneumonia group of avian origin: their classification into species. Ann NY Acad Sci 1960; 79:696–702
    [Google Scholar]
  7. Frey M.L., Hanson R.P., Anderson D.P. A medium for the isolation of avian mycoplasmas. Am J Net Res 1968; 29:2163–2171
    [Google Scholar]
  8. Garcia M., Elfaki M.G., Kleven S.H. Analysis of the variability in expression of Mycoplasma gallisepticum surface antigens. Vet Microbiol 1994; 42:147–158
    [Google Scholar]
  9. Glew M.D., Markham P.F., Browning G.F., Walker I.D. Expression studies on four members of the pMGA multigene family in Mycoplasma gallisepticum S6. Microbiology 1995; 141:3005–3014
    [Google Scholar]
  10. Gorton T.S., Goh M.S., Geary S.J. Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes. J Bacteriol 1995; 177:259–263
    [Google Scholar]
  11. Hanvey J.C., Klysik J., Wells R.D. Influence of DNA sequence on the formation of non-B right-handed helices in oligopurine-oligopyrimidine inserts in plasmids. J Biol Chem 1988; 263:7386–7396
    [Google Scholar]
  12. Kleven S.H., Browning G.F., Bulach D.M., Ghiocas E., Morrow C.J., Whithear K.G. Examination of Mycoplasma gallisepticum strains using restriction endonuclease DNA analysis and DNA-DNA hybridisation. Avian Pathol 1988; 17:559–570
    [Google Scholar]
  13. Kleven S.H., Morrow C.J., Whithear K.G. Comparison of Mycoplasma gallisepticum strains by hemagglutination-inhibition and restriction endonuclease analysis. Avian Dis 1988; 32:731–741
    [Google Scholar]
  14. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol 1990; 212:579–598
    [Google Scholar]
  15. Markham P.F. The pMGA gene repertoire of Mycoplasma gallisepticum 1995 PhD thesis, University of Melbourne;
    [Google Scholar]
  16. Markham P.F., Glew M., D.; Brandon M.R., Walker I.D., Whithear K.G. Characterization of a major hemagglutinin protein from Mycoplasma gallisepticum. Infect Immun 1992; 60:3885–3891
    [Google Scholar]
  17. Markham P.F., Glew M.D., Whithear K.G., Walker I.D. Molecular cloning of a member of the gene family that encodes pMGA, a hemagglutinin of Mycoplasma gallisepticum. Infect Immun 1993; 61:903–909
    [Google Scholar]
  18. Markham P.F., Glew M.D., Sykes J.E., Bowden T.R., Pollocks T.D., Browning G.F., Whithear K.G., Walker I.D. The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum. FEBS Lett 1994; 352:347–352
    [Google Scholar]
  19. Soeripto Whithear K.G., Cottew G.S., Harrigan K.E. Virulence and transmissibility of Mycoplasma gallisepticum. Aust Vet J 1989; 66:65–72
    [Google Scholar]
  20. Tigges E., Minion F.C. Physical map of Mycoplasma gallisepticum. J Bacteriol 1994; 176:4157–4159
    [Google Scholar]
  21. Whithear K.G., Soeripto Harrigan K.E., Ghiocas E. Safety of temperature sensitive mutant Mycoplasma gallisepticum vaccine. Aust Vet J 1990; 67:159–165
    [Google Scholar]
  22. Wise K.S. Adaptive surface variation in mycoplasmas. Trends Microbiol 1993; 1:59–63
    [Google Scholar]
  23. Yogev D., Menaker D., Strutzberg K., Levisohn S., Kirchhoff H., Hinz K.-H., Rosengarten R. A surface epitope undergoing high-frequency phase variation is shared by Mycoplasma gallisepticum and Mycoplasma bovis. Infect Immun 1994; 62:4962–4968
    [Google Scholar]
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