1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 6

gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field Free

Abstract

An iron-reducing, moderately thermophilic, acidophilic actinobacterium, strain IC-180, isolated from a solfataric field in Hakone, Japan, was subjected to polyphasic taxonomic analysis. Strain IC-180 was a motile, short rod-shaped, Gram-positive bacterium that was able to grow at temperatures of 35–58 °C (optimally at 50 °C) and at pH 2.0–4.5 (optimally at pH 3.0). The strain grew aerobically and heterotrophically. It also grew anaerobically or autotrophically by dissimilatory reduction of ferric iron. No oxidation of ferrous iron was observed. Major cellular fatty acids detected were iso-C, anteiso-C and iso-C; the major menaquinone was MK-9(H). Phosphatidyl--methylethanolamine and an unknown ninhydrin-positive phosphoglycolipid were detected. The total DNA G+C content was 74.1 mol%. 16S rRNA gene sequence comparisons revealed that strain IC-180 was a member of the order and clustered coherently with uncultured actinobacteria from a geothermal site and a bioreactor operated under moderately thermophilic conditions. This cluster could be distinguished from the two other clusters comprising the families of this order, and , respectively. Based on the properties of strain IC-180 determined in this polyphasic taxonomic study, this strain represents a novel species in a new genus in the order , for which the name gen. nov., sp. nov. is proposed; the type strain is IC-180 ( = JCM 15389  = DSM 45281).

  • Published Online:
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 13660338)
© 2011 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023044-0
2011-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/6/1281.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023044-0&mimeType=html&fmt=ahah

References

  1. Bridge T. A. M., Johnson D. B. 1998; Reduction of soluble iron and reductive dissolution of ferric iron-containing minerals by moderately thermophilic iron-oxidizing bacteria. Appl Environ Microbiol 64:2181–2186[PubMed]
     [Google Scholar]
  2. Brierley J. A. 1978; Thermophilic iron-oxidizing bacteria found in copper leaching dumps. Appl Environ Microbiol 36:523–525[PubMed]
     [Google Scholar]
  3. Brock T. D. 1986; Introduction: an overview of the thermophiles. In Thermophiles: General, Molecular and Applied Microbiology pp. 1–16 Edited by Brock T. D. New York: Wiley;
     [Google Scholar]
  4. Brock T. D., Brock K. M., Belly R. T., Weiss R. L. 1972; Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol 84:54–68 [View Article][PubMed]
     [Google Scholar]
  5. Clark D. A., Norris P. R. 1996; Acidimicrobium ferrooxidans gen. nov., sp. nov.: mixed-culture ferrous iron oxidation with Sulfobacillus species. Microbiology 142:785–790 [View Article]
     [Google Scholar]
  6. Davis-Belmar C. S., Norris P. R. 2009; Ferrous iron and pyrite oxidation by “Acidithiomicrobium” species. Adv Matr Res 71–73:271–274 [View Article]
     [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
     [Google Scholar]
  8. Itoh T., Yoshikawa N., Takashina T. 2007; Thermogymnomonas acidicola gen. nov., sp. nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan. Int J Syst Evol Microbiol 57:2557–2561 [View Article][PubMed]
     [Google Scholar]
  9. Johnson D. B. 2007; Physiology and ecology of acidophilic microorganisms. In Physiology and Biochemistry of Extremophiles pp. 257–270 Edited by Gerday C., Glansdorff N. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  10. Johnson D. B., Okibe N., Roberto F. F. 2003; Novel thermo-acidophilic bacteria isolated from geothermal sites in Yellowstone National Park: physiological and phylogenetic characteristics. Arch Microbiol 180:60–68 [View Article][PubMed]
     [Google Scholar]
  11. Johnson D. B., Bacelar-Nicolau P., Okibe N., Thomas A., Hallberg K. B. 2009; Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria. Int J Syst Evol Microbiol 59:1082–1089 [View Article][PubMed]
     [Google Scholar]
  12. Kurahashi M., Fukunaga Y., Sakiyama Y., Harayama S., Yokota A. 2009; Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.. Int J Syst Evol Microbiol 59:869–873 [View Article][PubMed]
     [Google Scholar]
  13. Lovley D. R., Phillips E. J. P. 1986; Availability of ferric iron for microbial reduction in bottom sediments of the freshwater tidal Potomac River. Appl Environ Microbiol 52:751–757[PubMed]
     [Google Scholar]
  14. Matsumoto A., Kasai H., Matsuo Y., Ōmura S., Shizuri Y., Takahashi Y. 2009; Ilumatobacter fluminis gen. nov., sp. nov., a novel actinobacterium isolated from the sediment of an estuary. J Gen Appl Microbiol 55:201–205 [View Article][PubMed]
     [Google Scholar]
  15. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  16. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T. 2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55:315–320 [View Article][PubMed]
     [Google Scholar]
  17. Phillips E. J. P., Lovley D. R. 1987; Determination of Fe(III) and Fe(II) in oxalate extracts of sediment. Soil Sci Soc Am J 51:938–941 [View Article]
     [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  19. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp. 463–470 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  21. Zhi X.-Y., Li W.-J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023044-0
Loading
Aciditerrimonas ferrireducens gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field
Int J Syst Evol Microbiol 61, 1281 (2011); https://doi.org/10.1099/ijs.0.023044-0
/content/journal/ijsem/10.1099/ijs.0.023044-0
/content/journal/ijsem/10.1099/ijs.0.023044-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error