1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 6

sp. nov., isolated from cow milk cheese in Nigeria No Access

Abstract

During microbial assessment of cow milk cheese products in the city of Ilorin, Nigeria, a -like isolate was detected that could not be assigned to any known species. Whole-genome sequence analyses against all currently known 26 species confirmed that this isolate constitutes a new taxon within the genus , with highest similarity to (average nucleotide identity of 82.66%, DNA–DNA hybridization of 28.3%). Phenotypically, it differs from by the inability to ferment sucrose, -fucose and starch. The absence of haemolysis and pathogenic islands suggest that this novel species is not pathogenic for humans and animals. The name sp. nov. is proposed, with the type strain CLIP 2019/01311 (=CIP 111875=DSM 111566).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AAI , ANI , Firmicutes , isDDH , new taxa and whole genome sequencing
Funding
This study was supported by the:
  • Santé Publique France
    • Principle Award Recipient: NotApplicable
  • Inserm
    • Principle Award Recipient: NotApplicable
  • Institut Pasteur
    • Principle Award Recipient: NotApplicable
  • University of Ilorin (Award 2017 N.G. Patel Grant Award)
    • Principle Award Recipient: IbrahimAdisa Raufu
© 2022 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005437
2022-06-22
2024-04-23
Loading full text...

Full text loading...

References

  1. Yong K-T, Ding H, Roy I, Law W-C, Bergey EJ et al. Imaging pancreatic cancer using bioconjugated InP quantum dots. ACS Nano 2009; 3:502–510 [View Article] [PubMed]
     [Google Scholar]
  2. Orsi RH, Wiedmann M. Characteristics and distribution of Listeria spp., including Listeria species newly described since 2009. Appl Microbiol Biotechnol 2016; 100:5273–5287 [View Article] [PubMed]
     [Google Scholar]
  3. Chiara M, Caruso M, D’Erchia AM, Manzari C, Fraccalvieri R et al. Comparative genomics of Listeria sensu lato: genus-wide differences in evolutionary dynamics and the progressive gain of complex, potentially pathogenicity-related traits through lateral gene transfer. Genome Biol Evol 2015; 7:2154–2172 [View Article] [PubMed]
     [Google Scholar]
  4. Pirie JH. The genus Listerella Pirie. Science 1940; 91:383 [View Article] [PubMed]
     [Google Scholar]
  5. Seeliger HPR. Apathogene listerien: L. innocua sp.n. zentralblatt für bakteriol mikrobiol und hyg 1 abt orig A. Medizinische Mikrobiol Infekt und Parasitol 1981 [View Article]
     [Google Scholar]
  6. Rocourt J, Grimont PAD. Notes: Listeria welshimeri sp. nov. and Listeria seeligeri sp. nov. Int J Syst Bacteriol 1983; 33:866–869 [View Article]
     [Google Scholar]
  7. Seeliger HPR, Rocourt J, Schrettenbrunner A, Grimont PAD, Jones D. Notes: Listeria ivanovii sp. nov. Int J Syst Bacteriol 1984; 34:336–337 [View Article]
     [Google Scholar]
  8. Graves LM, Helsel LO, Steigerwalt AG, Morey RE, Daneshvar MI et al. Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest. Int J Syst Evol Microbiol 2010; 60:1280–1288 [View Article] [PubMed]
     [Google Scholar]
  9. Carlin CR, Liao J, Weller DL, Guo X, Orsi R et al. Corrigendum: listeria cossartiae sp. nov., listeria farberi sp. nov., listeria immobilis sp. nov., listeria portnoyi sp. nov. and listeria rustica sp. nov., isolated from agricultural water and natural environments. Int J Syst Evol Microbiol 2021; 71:004795 [View Article]
     [Google Scholar]
  10. Larsen R, Seeliger HPR. A mannitol fermenting Listeria: Listeria grayi sp. n. In Larsen HE. eds Proceedings of the Third International Symposium on Listeriosis Bilthoven, The Netherlands: 1966
     [Google Scholar]
  11. Leclercq A, Clermont D, Bizet C, Grimont PAD, Le Flèche-Matéos A et al. Listeria rocourtiae sp. nov. Int J Syst Evol Microbiol 2010; 60:2210–2214 [View Article] [PubMed]
     [Google Scholar]
  12. Lang Halter E, Neuhaus K, Scherer S. Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond. Int J Syst Evol Microbiol 2013; 63:641–647 [View Article]
     [Google Scholar]
  13. den Bakker HC, Warchocki S, Wright EM, Allred AF, Ahlstrom C et al. Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments. Int J Syst Evol Microbiol 2014; 64:1882–1889 [View Article]
     [Google Scholar]
  14. Weller D, Andrus A, Wiedmann M, den Bakker HC. Listeria booriae sp. nov. and Listeria newyorkensis sp. nov., from food processing environments in the USA. Int J Syst Evol Microbiol 2015; 65:286–292 [View Article]
     [Google Scholar]
  15. Núñez-Montero K, Leclercq A, Moura A, Vales G, Peraza J et al. Listeria costaricensis sp. nov. Int J Syst Evol Microbiol 2018; 68:844–850 [View Article] [PubMed]
     [Google Scholar]
  16. Doijad SP, Poharkar KV, Kale SB, Kerkar S, Kalorey DR et al. Listeria goaensis sp. nov. Int J Syst Evol Microbiol 2018; 68:3285–3291 [View Article] [PubMed]
     [Google Scholar]
  17. Leclercq A, Moura A, Vales G, Tessaud-Rita N, Aguilhon C et al. Listeria thailandensis sp. nov. Int J Syst Evol Microbiol 2019; 69:74–81 [View Article] [PubMed]
     [Google Scholar]
  18. Quereda JJ, Leclercq A, Moura A, Vales G, Gómez-Martín Á et al. Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep. Int J Syst Evol Microbiol 2020; 70:5868–5879 [View Article] [PubMed]
     [Google Scholar]
  19. Guillet C, Join-Lambert O, Le Monnier A, Leclercq A, Mechaï F et al. Human listeriosis caused by Listeria ivanovii. . Emerg Infect Dis 2010; 16:136–138 [View Article] [PubMed]
     [Google Scholar]
  20. Thouvenot P, Vales G, Bracq-Dieye H, Tessaud-Rita N, Maury MM et al. MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study. J Microbiol Methods 2018; 144:29–32 [View Article] [PubMed]
     [Google Scholar]
  21. Moura A, Tourdjman M, Leclercq A, Hamelin E, Laurent E et al. Real-time whole-genome sequencing for surveillance of Listeria monocytogenes, France. Emerg Infect Dis 2017; 23:1462–1470 [View Article] [PubMed]
     [Google Scholar]
  22. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  23. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article] [PubMed]
     [Google Scholar]
  24. Robertson J, Nash JHE. MOB-suite: software tools for clustering, reconstruction and typing of plasmids from draft assemblies. Microb Genom 2018; 4: [View Article] [PubMed]
     [Google Scholar]
  25. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article] [PubMed]
     [Google Scholar]
  26. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015; 31:3691–3693 [View Article] [PubMed]
     [Google Scholar]
  27. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article] [PubMed]
     [Google Scholar]
  28. Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015; 32:268–274 [View Article] [PubMed]
     [Google Scholar]
  29. Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 2017; 14:587–589 [View Article] [PubMed]
     [Google Scholar]
  30. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  31. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 8:6–9
     [Google Scholar]
  32. Rodriguez-R LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. PeerJ Preprints 2016 [View Article]
     [Google Scholar]
  33. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
     [Google Scholar]
  34. Rodriguez-R LM, Konstantinidis KT. Bypassing cultivation to identify bacterial species. Microbe Magazine 2014; 9:111–118 [View Article]
     [Google Scholar]
  35. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  36. Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004; 42:3819–3822 [View Article] [PubMed]
     [Google Scholar]
  37. Moura A, Criscuolo A, Pouseele H, Maury MM, Leclercq A et al. Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes. . Nat Microbiol 2016; 2:16185 [View Article] [PubMed]
     [Google Scholar]
  38. Hitchins AD, Jinneman K, Chen Y. Detection of Listeria monocytogenes in foods and environmental samples, and enumeration of Listeria monocytogenes in foods. In Bacteriological Analytical Manual US Food and Drug Administration; 2016
     [Google Scholar]
  39. McLauchlin J, Reese CED. Genus Listeria. In Vos P, Garrity G, Jones D, Krieg NR. eds Bergey’s Manual of Systematic Bacteriology New York: Springer; 2009
     [Google Scholar]
  40. Bertsch D, Rau J, Eugster MR, Haug MC, Lawson PA et al. Listeria fleischmannii sp. nov., isolated from cheese. Int J Syst Evol Microbiol 2013; 63:526–532 [View Article] [PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005437
Loading
Listeria ilorinensis sp. nov., isolated from cow milk cheese in Nigeria
Int J Syst Evol Microbiol 72, 005437 (2022); https://doi.org/10.1099/ijsem.0.005437
/content/journal/ijsem/10.1099/ijsem.0.005437
/content/journal/ijsem/10.1099/ijsem.0.005437
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error