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Volume 72, Issue 12

gen. nov., sp. nov., an endophyte from a pine tree of the family , class No Access

Abstract

Bacterial strain A52C2 was isolated from the endophytic microbial community of a tree trunk and characterized. Strain A52C2 stained Gram-negative and formed rod-shaped cells that grew optimally at 30 °C and at pH 6.0–7.0. The G+C content of the DNA was 65.1 mol %. The respiratory quinone was ubiquinone 10, and the major fatty acids were cyclo-C 8 and C, representing 70.1 % of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain A52C2 in a distinct lineage within the order , family . The 16S rRNA gene sequence similarities of A52C2 to that of and were 93.15 and 93.2 %, respectively. The draft genome sequence of strain A52C2 comprises 4 196 045 bases with a 195-fold mapped coverage of the genome. The assembled genome consists of 43 contigs of more than 1 000 bp ( contig size was 209 720 bp). The genome encodes 4033 putative coding sequences. The phylogenetic, phenotypic and chemotaxonomic data showed that strain A52C2 (=UCCCB 130=CECT 8949=LMG 29042) represents the type of a novel species and genus, for which we propose the name gen. nov., sp. nov.

  • Received:
  • Accepted:
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Keyword(s): pine wilt disease , Alphaproteobacteria , endophyte , Faunimonas pinastri and genome
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award UID/EMS/00285/2020)
    • Principle Award Recipient: DiogoNeves Proença
  • Fundação para a Ciência e a Tecnologia (Award PTWPTDC/AAG-REC/3839/2014)
    • Principle Award Recipient: PaulaV. Morais
  • Horizon 2020 Framework Programme (Award 821096)
    • Principle Award Recipient: PaulaV. Morais
  • Office of Science (Award DE-AC02-05CH11231)
    • Principle Award Recipient: WilliamB. Whitman
© 2022 The Authors
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Faunimonas pinastri gen. nov., sp. nov., an endophyte from a pine tree of the family Pleomorphomonadaceae, class Alphaproteobacteria
Int J Syst Evol Microbiol 72, 005623 (2023); https://doi.org/10.1099/ijsem.0.005623
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