Abstract
Cromileptes altivelas that belongs to Serranidae in the order Perciformes, is widely distributed throughout the tropical waters of the Indo-West Pacific regions. Due to their excellent food quality and abundant nutrients, it has become a popular marine food fish with high market values. Here, we reported a chromosome-level genome assembly and annotation of the humpback grouper genome using more than 103X PacBio long-reads and high-throughput chromosome conformation capture (Hi-C) technologies. The N50 contig length of the assembly is as large as 4.14 Mbp, the final assembly is 1.07 Gb with N50 of scaffold 44.78 Mb, and 99.24% of the scaffold sequences were anchored into 24 chromosomes. The high-quality genome assembly also showed high gene completeness with 27,067 protein coding genes and 3,710 ncRNAs. This high accurate genome assembly and annotation will not only provide an essential genome resource for C. altivelas breeding and restocking, but will also serve as a key resource for studying fish genomics and genetics.
Competing Interest Statement
The authors have declared no competing interest.
Abbreviations
- BLAST
- Basic Local Alignment Search Tool
- BUSCO
- Benchmarking Universal Single-Copy Orthologs
- CEGMA
- Core Eukaryotic Genes Mapping Approach
- MITE
- miniature inverted–repeat transposable elements
- NCBI
- National Center for Biotechnology Information
- PacBio
- Pacific Biosciences
- PASA
- Program to Assemble Spliced Alignment
- PASA-T-set
- PASA Trinity set
- RNA-seq
- RNA sequencing