Abstract
Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at the fine scale in the mammalian brain. To address this question, we functionally mapped the spatial organization of the input axons and target neurons in the mouse retinocollicular pathway at single-cell resolution using in vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling of retinal ganglion cell axon terminals, with an average error below 30 μm or 2 degrees of visual angle. The precision of retinotopy was relatively lower for local neurons in the superior colliculus. Subsequent data-driven modelling ascribed it to a low input convergence, on average 5.5 retinal ganglion cell inputs to a postsynaptic cell in the superior colliculus. These results indicate that retinotopy arises largely from topographically precise input from presynaptic cells, rather than elaborating local circuitry to reconstruct the topography by postsynaptic cells.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Revised Figures and main text for clarification; and added another example of RGC axon tiling analysis in Supplementary Figure.
Acronyms
- 2D
- two-dimensional
- AAV
- adeno-associated virus
- CNMF
- constrained non-negative matrix factorization
- RF
- receptive field
- RGC
- retinal ganglion cells
- SC
- superior colliculus
- SD
- standard deviation